cholesteryl-arachidonate and cholesteryl-oleate

A reversed phase high performance liquid chromatographic (HPLC) method was developed for direct profiling and determination of adrenal cholesteryl ester composition. Cholesteryl adrenate and cholesteryl cervonate, which are not commercially available, were synthesized as markers. Lipid extracts of rat adrenal homogenates or lipid droplets were individually applied to a conditioned silica gel-60 column which separated cholesteryl esters from other native lipids. The eluted cholesteryl ester fraction was then analyzed by HPLC. With cholesteryl heptadecanoate as internal standard, seven adrenal cholesteryl esters were detected and quantified: cholesteryl cervonate, cholesteryl arachidonate, cholesteryl adrenate, cholesteryl myristate, cholesteryl oleate, cholesteryl palmitate, and cholesteryl stearate. Among them, cholesteryl adrenate appeared to be the major sterol ester stored in the rat adrenal.

Topics: Adrenal Glands; Animals; Arachidonic Acids; Cholesterol Esters; Chromatography, High Pressure Liquid; Male; Rats; Rats, Sprague-Dawley

Murine resident peritoneal macrophages were maintained in cell culture in a medium containing 10% lipoprotein-deficient fetal calf serum to which various artificial lipoprotein particles (coacervates of lipid and bovine serum albumin) had been added. The uptake and intracellular fate of these particles was studied by electron microscopy. The appearance of material accumulating within the cells varied according to the nature of the lipid component of the ingested particles. Lipids which are readily oxidised (cholesteryl linoleate, cholesteryl arachidonate, trilinolein) were associated with the formation of ceroid within membrane-bound structures. Less readily oxidized lipids (cholesteryl oleate, triolein) were not associated with ceroid accumulation but instead the cells contained numerous nonmembrane-bound lipid inclusions. The appearances of the ceroid within the murine peritoneal macrophages are similar to those of ceroid in macrophages in human atherosclerotic lesions.

Topics: Animals; Arachidonic Acids; Cells, Cultured; Ceroid; Cholesterol Esters; Lipoproteins; Macrophages; Male; Mice; Mice, Inbred BALB C; Microscopy, Electron; Phagocytosis; Phagosomes; Pigments, Biological; Triglycerides; Triolein

The blood serum of coronary and liver patients was shown to display a similar increase in the relative levels of cholesterololeate and a some what less marked elevation in the content of cholesterol esters with saturated fatty acids. The cholesterollinoleate concentration was enhanced in patients with viral hepatitis and decreased in coronary heart disease patients. The differences were especially distinct in changes of the relative content of cholesterolarachidonate and esters of cholesterol with eicosopentaen acid. Cholesterolarachidonate levels showed no changes in CHD whereas they lowered markedly in various diseases of the liver and biliary tracts. The role of changes in the relative content of cholesterol ester fractions in the genesis of atherosclerosis in patients with liver diseases is discussed.

Topics: Arachidonic Acids; Cholecystitis; Cholelithiasis; Cholesterol Esters; Coronary Disease; Fatty Acids; Hepatitis; Humans; Liver Cirrhosis

Topics: Arachidonic Acids; Cholesterol Esters; Humans; Kinetics; Lipoproteins, LDL; Magnetic Resonance Spectroscopy; Protein Binding; Protein Conformation; Temperature