cholecalciferol and 2-ethylidene-1-5-dimethyl-3-3-diphenylpyrrolidine

cholecalciferol has been researched along with 2-ethylidene-1-5-dimethyl-3-3-diphenylpyrrolidine* in 1 studies

Other Studies

1 other study(ies) available for cholecalciferol and 2-ethylidene-1-5-dimethyl-3-3-diphenylpyrrolidine

Article	Year
Evaluation of the influence of protein precipitation prior to on-line SPE-LC-API/MS procedures using multivariate data analysis. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007, Jan-15, Volume: 845, Issue:2 Matrix effects on mass spectrometry (MS) response were investigated with three atmospheric pressure ionization (API) sources after on-line solid-phase extraction (SPE) of human plasma. On-line SPE was evaluated with one restricted access material (RAM), two large particle supports (LPS) and one monolith. A sample protein precipitation (PP) with acetonitrile (2:1) and a direct injection were tested. Principal component analysis (PCA) was performed to simplify data presentation and interpretation. Protein precipitation was found to be mandatory for reducing signal modification. Regarding sensitivity towards matrix effects after PP, atmospheric pressure photoionization (APPI) was globally the least sensitive ionization mode while electrospray ionization ESI was the most sensitive. Topics: Alanine; Blood Proteins; Chemical Precipitation; Cholecalciferol; Chromatography, Liquid; Flunitrazepam; Fluoxetine; Mass Spectrometry; Methadone; Multivariate Analysis; Online Systems; Pyrrolidines; Solid Phase Extraction	2007

Article

Year

Evaluation of the influence of protein precipitation prior to on-line SPE-LC-API/MS procedures using multivariate data analysis.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007, Jan-15, Volume: 845, Issue:2

Matrix effects on mass spectrometry (MS) response were investigated with three atmospheric pressure ionization (API) sources after on-line solid-phase extraction (SPE) of human plasma. On-line SPE was evaluated with one restricted access material (RAM), two large particle supports (LPS) and one monolith. A sample protein precipitation (PP) with acetonitrile (2:1) and a direct injection were tested. Principal component analysis (PCA) was performed to simplify data presentation and interpretation. Protein precipitation was found to be mandatory for reducing signal modification. Regarding sensitivity towards matrix effects after PP, atmospheric pressure photoionization (APPI) was globally the least sensitive ionization mode while electrospray ionization ESI was the most sensitive.

Topics: Alanine; Blood Proteins; Chemical Precipitation; Cholecalciferol; Chromatography, Liquid; Flunitrazepam; Fluoxetine; Mass Spectrometry; Methadone; Multivariate Analysis; Online Systems; Pyrrolidines; Solid Phase Extraction

2007