chlortetracycline-hydrochloride and sulfomycin

Enhanced solvent extraction (ESE) was used to isolate components of alexomycin from different types of animal feed samples. It was demonstrated that ESE was more economical as regards money and, for this particular matrix, twice as fast as supercritical fluid extraction (SFE) even though it was demonstrated that alexomycin can be extracted from feed quantitatively using both ESE and SFE. Supercritical fluid chromatography was used to separate alexomycin from other co-extractives of feed since liquid chromatography was unable to isolate the alexomycin for quantification purposes. Our results also showed that the concentration of alexomycin in an extruded sample was approximately half of the concentration which was originally added to the feed.

Topics: Animal Feed; Anti-Bacterial Agents; Chromatography; Chromatography, High Pressure Liquid; Methanol; Peptides; Peptides, Cyclic; Propylamines; Time Factors

We have developed HPCE methods for the analysis of sulfomycin (trivial name) and related compounds (code name, crude material = U82127 = I), which is an animal growth promoter derived from a fermentation beer. The crude material, I, isolated from the fermentation consisted of more than 40 components which were not completely separated by conventional HPLC. Thus, as a complementary analysis method, we have optimized HPCE conditions for I using various capillaries including uncoated, coated, and packed using various buffers. The optimized HPCE conditions were obtained with an uncoated fused-silica capillary and a buffer that consisted of 30 mM Tris-tricine, 10 mM SDS, 10 mM NaCl and 20% MeOH, pH 8.0. Using these HPCE conditions, we were able to separate the one main component collected from the HPLC effluent into two or three components. In order to identify the main components of the fermentation product, an off-line HPLC-HPCE-MS analysis for I was performed. From the MALDI-TOF-MS results, the separated components collected from HPCE had different molecular masses. Four lots of I samples having different characteristics were also analyzed by HPCE to investigate lot-to-lot differences in peak profiles. The four lots of I were found to have very similar peak profiles. In this paper, I refers to the crude fermentation product and sulfomycin to the purified, major HPLC component of I.

Topics: Animals; Anti-Bacterial Agents; Beer; Buffers; Chromatography, High Pressure Liquid; Electrophoresis, Capillary; Fermentation; Growth Substances; Peptides; Peptides, Cyclic; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization