chlorophyll and chlorophyll-b

chlorophyll has been researched along with chlorophyll-b* in 21 studies

Reviews

1 review(s) available for chlorophyll and chlorophyll-b

ArticleYear
Meta-Analysis of the Detection of Plant Pigment Concentrations Using Hyperspectral Remotely Sensed Data.
    PloS one, 2015, Volume: 10, Issue:9

    Passive optical hyperspectral remote sensing of plant pigments offers potential for understanding plant ecophysiological processes across a range of spatial scales. Following a number of decades of research in this field, this paper undertakes a systematic meta-analysis of 85 articles to determine whether passive optical hyperspectral remote sensing techniques are sufficiently well developed to quantify individual plant pigments, which operational solutions are available for wider plant science and the areas which now require greater focus. The findings indicate that predictive relationships are strong for all pigments at the leaf scale but these decrease and become more variable across pigment types at the canopy and landscape scales. At leaf scale it is clear that specific sets of optimal wavelengths can be recommended for operational methodologies: total chlorophyll and chlorophyll a quantification is based on reflectance in the green (550-560nm) and red edge (680-750nm) regions; chlorophyll b on the red, (630-660nm), red edge (670-710nm) and the near-infrared (800-810nm); carotenoids on the 500-580nm region; and anthocyanins on the green (550-560nm), red edge (700-710nm) and near-infrared (780-790nm). For total chlorophyll the optimal wavelengths are valid across canopy and landscape scales and there is some evidence that the same applies for chlorophyll a.

    Topics: Anthocyanins; Carotenoids; Chlorophyll; Chlorophyll A; Light; Pigments, Biological; Plant Leaves; Plants; Publications; Remote Sensing Technology; Spectrum Analysis

2015

Other Studies

20 other study(ies) available for chlorophyll and chlorophyll-b

ArticleYear
The pigment binding behaviour of water-soluble chlorophyll protein (WSCP).
    Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2020, May-20, Volume: 19, Issue:5

    Water-soluble chlorophyll proteins (WSCPs) are homotetrameric proteins that bind four chlorophyll (Chl) molecules in identical binding sites, which makes WSCPs a good model to study protein-pigment interactions. In a previous study, we described preferential binding of Chl a or Chl b in various WSCP versions. Chl b binding is preferred when a hydrogen bond can be formed between the C7 formyl of the chlorin macrocycle and the protein, whereas Chl a is preferred when Chl b binding is sterically unfavorable. Here, we determined the binding affinities and kinetics of various WSCP versions not only for Chl a/b, but also for chlorophyllide (Chlide) a/b and pheophytin (Pheo) a/b. Altered KD values are responsible for the Chl a/b selectivity in WSCP whereas differences in the reaction kinetics are neglectable in explaining different Chl a/b preferences. WSCP binds both Chlide and Pheo with a lower affinity than Chl, which indicates the importance of the phytol chain and the central Mg2+ ion as interaction sites between WSCP and pigment. Pheophorbide (Pheoide), lacking both the phytol chain and the central Mg2+ ion, can only be bound as Pheoide b to a WSCP that has a higher affinity for Chl b than Chl a, which underlines the impact of the C7 formyl-protein interaction. Moreover, WSCP was able to bind protochlorophyllide and Mg-protoporphyrin IX, which suggests that neither the size of the π electron system of the macrocycle nor the presence of a fifth ring at the macrocycle notably affect the binding to WSCP. WSCP also binds heme to form a tetrameric complex, suggesting that heme is bound in the Chl-binding site.

    Topics: Brassica; Chlorophyll; Lepidium; Light-Harvesting Protein Complexes; Pisum sativum; Plant Proteins; Protein Binding; Solubility; Water

2020
Safflower (
    Journal of agricultural and food chemistry, 2020, Jun-03, Volume: 68, Issue:22

    The steroid hormones, including brassinosteroids, regulate plant growth under stress. It is hypothesized that 24-epibrassinosteroids (24-EBR) can affect safflower (

    Topics: Carotenoids; Carthamus tinctorius; Chlorophyll; Droughts; Genotype; Malondialdehyde; Peroxidase; Plant Growth Regulators; Plant Oils; Plant Proteins; Seeds; Steroids; Superoxide Dismutase

2020
Sewage contamination under water scarcity effects on stream biota: biofilm, grazers, and their interaction.
    Environmental science and pollution research international, 2019, Volume: 26, Issue:26

    One of the most common anthropogenic impacts on river ecosystems is the effluent discharge from wastewater treatment plants. The effects of this contamination on stream biota may be intensified in Mediterranean climate regions, which comprise a drought period that leads to flow reduction, and ultimately to stagnant pools. To assess individual and combined effects of flow stagnation and sewage contamination, biofilm and gastropod grazers were used in a 5-week experiment with artificial channels to test two flow velocity treatments (stagnant flow/basal flow) and two levels of organic contamination using artificial sewage (no sewage input/sewage input). Stressors' effects were determined on biofilm total biomass and chlorophyll (Chl) content, on oxygen consumption and growth rate of the grazers (Theodoxus fluviatilis), and on the interaction grazer-biofilm given by grazer's feeding activity (i.e., biofilm consumption rate). The single effect of sewage induced an increase in biofilm biomass and Chl-a content, simultaneously increasing both grazers' oxygen consumption and their feeding activity. Diatoms showed a higher sensitivity to flow stagnation, resulting in a lower content of Chl-c. Combined stressors interacted antagonistically for biofilm total biomass, Chl-b contents, and grazers's feeding rate. The effect of sewage increasing biofilm biomass and grazing activity was reduced by the presence of flow stagnation (antagonist factor). Our findings suggest that sewage contamination has a direct effect on the functional response of primary producers and an indirect effect on primary consumers, and this effect is influenced by water flow stagnation.

    Topics: Biofilms; Biomass; Biota; Chlorophyll; Conservation of Water Resources; Diatoms; Ecosystem; Rhodophyta; Rivers; Sewage; Waste Disposal Facilities; Water Pollutants; Water Supply

2019
Responses of Hydrilla verticillata (L.f.) Royle and Vallisneria natans (Lour.) Hara to glyphosate exposure.
    Chemosphere, 2018, Volume: 193

    Glyphosate is a broad-spectrum herbicide that is frequently detected in water bodies and is harmful to aquatic systems. We conducted an experiment to explore the ecological sensitivity of Hydrilla verticillata (L.f.) Royle and Vallisneria natans (Lour.) Hara to glyphosate. Our research focused on the physiological responses of H. verticillata and V. natans after exposure to various concentrations of glyphosate (0, 1, 10, 20, 30, 40, 50 and 80 mg/L) in hydroponic culture after one day (1D) and seven days (7D). The results show that after 1D, the soluble protein content of H. verticillata was significantly stimulated under low herbicide concentrations. Other indices for H. verticillata and V. natans had no remarkable changes at 1D. After 7D of treatment, the soluble protein content of H. verticillata showed no significant differences, while the malondialdehyde (MDA), pigment contents and catalase (CAT) activity significantly increased at low glyphosate concentrations. Guaiacol peroxidase (POD) activity in H. verticillata significantly increased with increasing herbicide concentrations. The chlorophyll a/b ratio of H. verticillata sharply decreased above 10 mg/L. For V. natans, soluble protein, chlorophyll a, and carotenoid content; and CAT activity declined significantly after glyphosate application, while other indicators showed no significant changes. Our results indicate that glyphosate concentrations from 0 to 80 mg/L can induce oxidative stress in H. verticillate and may impede metabolism processes for protein and pigments without causing oxidative stress in V. natans. Taken together, our results suggest that the sensitivity of H. verticillata to glyphosate exposure is higher than that of V. natans.

    Topics: Animals; Catalase; Chlorophyll; Chlorophyll A; Glycine; Glyphosate; Herbicides; Hydrocharitaceae; Malondialdehyde; Oxidative Stress

2018
Impact of lead tolerant plant growth promoting rhizobacteria on growth, physiology, antioxidant activities, yield and lead content in sunflower in lead contaminated soil.
    Chemosphere, 2018, Volume: 195

    Present study was conducted to evaluate the effect of lead tolerant plant growth promoting rhizobacteria (LTPGPR) on growth, physiology, yield, antioxidant activities and lead uptake in sunflower in soil contaminated with lead under pot conditions. Three pre-characterized LTPGP strains (S2 (Pseudomonas gessardii strain BLP141), S5 (Pseudomonas fluorescens A506) and S10 (Pseudomonas fluorescens strain LMG 2189)) were used to inoculate sunflower growing in soil contaminated with different levels (300, 600 and 900 mg kg

    Topics: Antioxidants; Ascorbate Peroxidases; Catalase; Chlorophyll; Chlorophyll A; Environmental Pollution; Glutathione Reductase; Helianthus; Lead; Nitrates; Plant Development; Plant Roots; Proline; Pseudomonas; Soil; Soil Microbiology; Soil Pollutants; Superoxide Dismutase

2018
Reaction of Spring Barley and Common Radish on the Introduction of Ionic Liquids Containing Asymmetric Cations to the Soil.
    Journal of agricultural and food chemistry, 2017, Jun-14, Volume: 65, Issue:23

    The harmful effect of ionic liquids (ILs) on the environment is one of the important elements of scientific research conducted around the world. This study presents the effect of ionic liquids, containing the asymmetric cations benzyltrimethylammonium chloride [BenzTMA][Cl] and benzyltriethylammonium chloride [BenzTEA][Cl], on physiological and biochemical changes in common radish plants and spring barley seedlings. The examined ILs demonstrated low toxicity to higher plants. The compound that exhibited higher phytotoxicity to these plant species was [BenzTMA][Cl], whereas the plant that was more resistant to such ILs was common radish. Both the ionic liquids, particularly at higher concentrations, led to changes in the metabolism of plants, which resulted in a decrease of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids content. The observed changes were positively correlated with increasing concentrations of the examined ILs in the soil. In the case of spring barley, a decrease in the fresh weight and an increase in the dry weight of the seedlings were also observed. The evidence of oxidative stress occurrence in spring barley was observed due to the accumulation of malondialdehyde and free proline, as well as due to an increase in the activity of catalase and peroxidase. The changes in these biomarkers indicating oxidative stress occurrence in common radish plants were much lower. An increase in the content of chloride ions was observed in both the plants.

    Topics: Carotenoids; Cations; Chlorophyll; Hordeum; Ionic Liquids; Malondialdehyde; Oxidative Stress; Raphanus; Soil Pollutants

2017
Effect of Cuscuta campestris parasitism on the physiological and anatomical changes in untreated and herbicide-treated sugar beet.
    Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 2017, Nov-02, Volume: 52, Issue:11

    The effects of field dodder on physiological and anatomical processes in untreated sugar beet plants and the effects of propyzamide on field dodder were examined under controlled conditions. The experiment included the following variants: N-noninfested sugar beet plants (control); I - infested sugar beet plants (untreated), and infested plants treated with propyzamide (1500 g a.i. ha

    Topics: Animals; Benzamides; Beta vulgaris; Chlorophyll; Chlorophyll A; Cuscuta; Herbicides; Photosynthesis; Plant Leaves

2017
Changes in gametophyte physiology of Pteris multifida induced by the leaf leachate treatment of the invasive Bidens pilosa.
    Environmental science and pollution research international, 2016, Volume: 23, Issue:4

    In recent years, the response of fern gametophytes to environment has raised much attention. However, studies on the influence of plant invasion to fern gametophytes are scarce. Allelopathy plays an important role in biological invasion. Hence, it is necessary to study the allelopathic effects of invasive plants on fern gametophytes and elucidate the mechanisms by which invasive plants cause phytotoxicity. As one of the main invasive plants in China, Bidens pilosa exhibits allelopathic effects on spermatophyte growth. Field investigation shows that many ferns are threatened by the invasion of B. pilosa. The distribution of Pteris multifida overlaps with that of B. pilosa in China. To examine the potential involvement of allelopathic mechanisms of B. pilosa leaves, changes in the physiology in P. multifida gametophytes are analyzed. We found that cell membrane and antioxidant enzyme activities as well as photosynthesis pigment contents of the gametophytes were affected by B. pilosa leachates. Gametophytes of P. multifida exposed to B. pilosa had increased damages to cell membranes, expressed in thiobarbituric acid reacting substance (TBARS) concentrations, malondialdehyde (MDA), electrolyte leakage (membrane permeability), and degree of injury. Enzyme activities, assessed by superoxide dismutase (SOD) and catalase (CAT) as well as guaiacol peroxidase (GPX) enhanced with the increase in leachate concentration after 2-day exposure. Meanwhile, lower chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoid (Car), and the total chlorophyll were measured as leachate concentrations increased. At day 10, leaf leachates of B. pilosa exhibited the greatest inhibition. These results suggest that the observed inhibitory or stimulatory effects on the physiology studied can have an adverse effect on P. multifida and that allelopathic interference seems to have involved in this process.

    Topics: Allelopathy; Bidens; Catalase; China; Chlorophyll; Chlorophyll A; Germ Cells, Plant; Introduced Species; Malondialdehyde; Pheromones; Photosynthesis; Plant Leaves; Pteris; Superoxide Dismutase

2016
Morphological and biochemical changes in Azadirachta indica from coal combustion fly ash dumping site from a thermal power plant in Delhi, India.
    Ecotoxicology and environmental safety, 2016, Volume: 129

    The foliar and biochemical traits of Azadirachta indica A. Juss from fly ash (FA) dumping site in Badarpur thermal power plant (BTPP) New Delhi, India was studied. Three different experimental sites were selected at different distances from the thermal power plant. Ambient suspended particulate matter (SPM) and plant responses such as leaf pigments (chlorophyll a, chlorophyll b, and carotenoids), total chlorophyll, net photosynthetic rate, stomatal index (SI), stomatal conductance (SC), intercellular carbon dioxide concentration [CO2]i, net photosynthetic rate (NPR), nitrogen, nitrate, nitrate reductase activity, proline, protein, reducing sugar and sulphur content were measured. Considerable reduction in pigments (chlorophyll a, chlorophyll b and carotenoids), and total chlorophyll was observed at fly ash dumping site. Fly ash stress revealed the inhibitory effect on Nitrate reductase activity (NRA), Nitrate, soluble protein, and reducing sugar content, whereas stimulatory effect was found for the stomatal index, nitrogen, proline, antioxidants and sulphur content in the leaves. Under fly ash stress, stomatal conductance was low, leading to declining in photosynthetic rate and increase in the internal CO2 concentration of leaf. Single leaf area (SLA), leaf length and leaf width also showed a declining trend from control to the polluted site. Antioxidant enzymes increased in leaves reflecting stress and extenuation of reactive oxygen species (ROS).

    Topics: Air Pollutants; Antioxidants; Azadirachta; Carbon Dioxide; Carotenoids; Chlorophyll; Chlorophyll A; Coal; Coal Ash; India; Nitrogen; Oxidative Stress; Photosynthesis; Plant Leaves; Power Plants; Proline; Sulfur

2016
Industrial dust sulphate and its effects on biochemical and morphological characteristics of Morus (Morus alba) plant in NCR Delhi.
    Environmental monitoring and assessment, 2015, Volume: 187, Issue:3

    Abundance of CaCO3 rich soil dust is a typical feature of atmospheric environment in the Indian region. During prevailing dry weather conditions, dustfall is deposited onto the foliar surfaces of plant affecting their morphology, stomata and the levels of biochemical constituents. This study reports the chemical characteristics of dustfall, its effect on foliar morphology and biochemical constituents of a medicinal plant (Morus alba) at two sites which are differentiated on the basis of landuse pattern, viz., (i) residential, Jawaharlal Nehru University (JNU), and (ii) industrial, Sahibabad (SB), located in the National Capital Region (NCR) of Delhi. Dustfall was characterized for major anions (F(-), Cl(-), NO3 (-) and SO4 (--)) and cations (Na(+), NH4 (+), K(+), Mg(++) and Ca(++)). Biochemical parameters such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, proline and ascorbic acid were determined in foliar samples. The results showed that the dustfall fluxes of all the major ions were found to be higher at the industrial site (SB) as compared to the residential site (JNU). Foliar analysis revealed that the levels of biochemical parameters were more affected at SB site due to higher levels of dust SO4 (--) contributed by various anthropogenic sources resulting in more stressful conditions affecting the biochemistry of the plant. The possible entry pathways for dust SO4 (--) into foliar cells are also discussed in the paper. It was noticed that the deposition of urban dust was responsible for the damage of trichome, epidermis, cuticle and stomatal guard cells significantly affecting foliar morphology. SB exhibited more damage to these morphological parts suggesting that industrial dust is harmful to the plants.

    Topics: Air Pollutants; Chlorophyll; Chlorophyll A; Dust; Environmental Monitoring; Industry; Ions; Morus; Plants; Soil; Sulfates

2015
Quantitative proteomic analysis of the rice (Oryza sativa L.) salt response.
    PloS one, 2015, Volume: 10, Issue:3

    Salt stress is one of most serious limiting factors for crop growth and production. An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach was used to analyze proteomic changes in rice shoots under salt stress in this study. A total of 56 proteins were significantly altered and 16 of them were enriched in the pathways of photosynthesis, antioxidant and oxidative phosphorylation. Among these 16 proteins, peroxiredoxin Q and photosystem I subunit D were up-regulated, while thioredoxin M-like, thioredoxin x, thioredoxin peroxidase, glutathione S-transferase F3, PSI subunit H, light-harvesting antenna complex I subunits, chloroplast chaperonin, vacuolar ATP synthase subunit H, and ATP synthase delta chain were down-regulated. Moreover, physiological data including total antioxidant capacity, peroxiredoxin activity, chlorophyll a/b content, glutathione S-transferase activity, reduced glutathione content and ATPase activity were consistent with changes in the levels of these proteins. The levels of the mRNAs encoding these proteins were also analyzed by real-time quantitative reverse transcription PCR, and approximately 86% of the results were consistent with the iTRAQ data. Importantly, our data suggest the important role of PSI in balancing energy supply and ROS generation under salt stress. This study provides information for an improved understanding of the function of photosynthesis and PSI in the salt-stress response of rice.

    Topics: Chlorophyll; Computational Biology; Gene Expression Regulation, Plant; Molecular Sequence Annotation; Oryza; Oxidative Phosphorylation; Oxidative Stress; Plant Proteins; Proteome; Proteomics; Salt Tolerance; Stress, Physiological

2015
Effects of Pb(Ⅱ) exposure on Chlorella protothecoides and Chlorella vulgaris growth, malondialdehyde, and photosynthesis-related gene transcription.
    Environmental toxicology, 2014, Volume: 29, Issue:11

    Greater exposure to Pb(Ⅱ) increases the likelihood of harmful effects in the environment. In this study, the aquatic unicellular alga Chlorella protothecoides (C. protothecoides) and Chlorella vulgaris (C. vulgaris) were chosen to assess the acute and chronic toxicity of Pb(Ⅱ) exposure. Results of the observations show dose-response relationships could be clearly observed between Pb(Ⅱ) concentration and percentage inhibition (PI). Exposure to Pb(Ⅱ) increased malondialdehyde (MDA) content by up to 4.22 times compared with the control, suggesting that there was some oxidative damage. ANOVA analysis shows that Pb(Ⅱ) decreased chlorophyll (chl) content, indicating marked concentration-dependent relationships, and the lowest levels of chl a, chl b, and total-chl were 14.53, 18.80, and 17.95% of the controls, respectively. A real-time PCR assay suggests the changes in transcript abundances of three photosynthetic-related genes. After 120 h exposure Pb(Ⅱ) reduced the transcript abundance of rbcL, psaB, and psbC, and the relative abundances of the three genes of C. protothecoides and C. vulgaris in response to Pb(Ⅱ) were 54.66-98.59, 51.68-95.59, 37.89-95.48, 36.04-94.94, 41.19-91.20, and 58.75-96.80% of those of the controls, respectively. As for 28 d treatments, the three genes displayed similar inhibitory trend. This research provides a basic understanding of Pb(Ⅱ) toxicity to aquatic organisms.

    Topics: Chlorella; Chlorella vulgaris; Chlorophyll; Chlorophyll A; Environmental Pollutants; Lead; Malondialdehyde; Oxidation-Reduction; Photosynthesis; Species Specificity; Transcription, Genetic

2014
Growth and metal removal potential of a Phormidium bigranulatum-dominated mat following long-term exposure to elevated levels of copper.
    Environmental science and pollution research international, 2014, Volume: 21, Issue:17

    The present study explores the tolerance and metal removal response of a well-developed 2-week-old Phormidium mat after long-term exposure to Cu(2+)-enriched medium. Cu(2+) enrichment inhibited increase in mat biomass in a concentration-dependent manner. Mat area and the number of entrapped air bubbles decreased as Cu(2+) concentration increased in the medium. Decrease in number of air bubbles obviously reflects the adverse effect of Cu(2+) on photosynthetic performance of the mat. Metal enrichment did not substantially alter the amount of pigments, such as chlorophyll a, chlorophyll b, carotenoids, and phycocyanin, in the mat. Enhancement of Cu(2+) concentration in the medium led to changes in species composition of the test mat; however, Phormidium bigranulatum always remained the dominant organism. Relative share of green algae and some cyanobacterial taxa, namely, Lyngbya sp. and Oscillatoria tenuis, in the mat were increased by Cu(2+) enrichment. The mat successfully removed 80 to 94 % Cu(2+) from the growth medium containing 10 to 100 μM Cu(2+). Extracellular polysaccharides, whose share increased in the mat community after metal addition, seem to have contributed substantially to metal binding by the mat biomass.

    Topics: Biodegradation, Environmental; Biomass; Chlorophyll; Chlorophyll A; Chlorophyta; Copper; Cyanobacteria; Metals; Photosynthesis; Toxicity Tests, Chronic

2014
Photochemical performance of thylakoid membrane in lead-treated Nymphoides peltatum.
    Bulletin of environmental contamination and toxicology, 2014, Volume: 93, Issue:2

    Photochemical responses in the thylakoid membrane of Nymphoides peltatum to increasing lead (Pb) concentrations were investigated after 5 days of exposure. Pb accumulation increased in a concentration-dependent manner, with a maximum of 118.44 μg g(-1) at 100 μM. Nutrients (Zn, Mg, Mo, Ca, Fe and Mn), ATPase activity and pigment generally increased progressively at Pb concentrations of 12.5 and 25 μM, but then declined at concentrations of 50 and 100 μM. Moreover, Pb stress induced an increase in chlorophyll (Chl) a/b ratio in a different extent. No outstanding changes were observed in several Chl a fluorescence parameters at low Pb concentrations (12.5 and 25 μM), while significant changes (p < 0.05) were observed in these parameters at higher concentrations. The alterations of nutrients, ATPase activity and pigment content were associated with disturbances in the thylakoid membrane, indicated by the quenching of Chl a fluorescence. These results were indicative of a disarray in photochemical activities exerted by Pb phytotoxicity.

    Topics: Chlorophyll; Chlorophyll A; Environmental Pollutants; Lead; Magnoliopsida; Thylakoids

2014
Enantioselective toxicity and degradation of the chiral insecticide fipronil in Scenedesmus obliguus suspension system.
    Environmental toxicology and chemistry, 2014, Volume: 33, Issue:11

    Fipronil is an effective insecticide, but it presents highly toxic effects in nontarget aquatic organisms. The present study examined the enantioselective toxicity and degradation of fipronil enantiomers in a freshwater algae Scenedesmus obliguus suspension. There was a substantial difference in the acute toxicity of the enantiomers to S. obliguus, with 72-h median effective concentrations (EC50s) of 0.29 mg L(-1) and 1.50 mg L(-1) for the R-fipronil and S-fipronil, respectively. The influences on the concentration of chlorophyll a, chlorophyll b, and carotenoids were determined, and the effects of fipronil on the concentration of chlorophyll a and chlorophyll b were also enantioselective. The degradation of fipronil in algae suspension was enantioselective, with half-lives for R-fipronil and S-fipronil of 2.9 d and 3.2 d, respectively, and the enantiomer fraction reaching 0.65 at the day 17. The enantiomeric differences should be taken into consideration for fipronil risk assessment.

    Topics: Biological Assay; Carotenoids; Chlorophyll; Chlorophyll A; Insecticides; Photosynthesis; Pyrazoles; Reproducibility of Results; Risk Assessment; Scenedesmus; Stereoisomerism; Suspensions; Time Factors

2014
Determination of chlorophylls in Taraxacum formosanum by high-performance liquid chromatography-diode array detection-mass spectrometry and preparation by column chromatography.
    Journal of agricultural and food chemistry, 2012, Jun-20, Volume: 60, Issue:24

    Taraxacum formosanum, a well-known Chinese herb shown to be protective against hepatic cancer as well as liver and lung damage, may be attributed to the presence of abundant carotenoids and chlorophylls. However, the variety and content of chlorophylls remain uncertain. The objectives of this study were to develop an high-performance liquid chromatography-diode array detection-mass spectrometry method for determination of chlorophylls in T. formosanum and preparation by column chromatography. An HyPURITY C18 column and a gradient mobile phase of water (A), methanol (B), acetonitrile (C), and acetone (D) could resolve 10 chlorophylls and an internal standard Fast Green FCF within 30 min with a flow rate at 1 mL/min and detection at 660 nm. Both chlorophylls a and a' were present in the largest amount (1389.6 μg/g), followed by chlorophylls b and b' (561.2 μg/g), pheophytins a and a' (31.7 μg/g), hydroxychlorophyll b (26.5 μg/g), hydroxychlorophylls a and a' (9.8 μg/g), and chlorophyllides a and a' (0.35 μg/g). A glass column containing 52 g of magnesium oxide-diatomaceous earth (1:3, w/w) could elute chlorophylls with 800 mL of acetone containing 50% ethanol at a flow rate of 10 mL/min. Some new chlorophyll derivatives including chlorophyllide b, pyropheophorbide b, hydroxypheophytin a, and hydroxypheophytin a' were generated during column chromatography but accompanied by a 63% loss in total chlorophylls. Thus, the possibility of chlorophyll fraction prepared from T. formosanum as a raw material for future production of functional food needs further investigation.

    Topics: Chlorophyll; Chlorophyll A; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Mass Spectrometry; Pheophytins; Taraxacum

2012
Chlorophyll a appearance in the dark in higher plants: analytical notes.
    Science (New York, N.Y.), 1963, Apr-05, Volume: 140, Issue:3562

    The increase in the ratio [chlorophyll a]/[chlorophyll b] which occurs in expanding bean leaves in the dark, is a result of chlorophyll a formation with a concomitant loss in chlorophyll b. The analytical methods for assay of these pigments were examined closely and found to be adequate for this purpose when proper safeguards are taken.

    Topics: Chlorophyll; Chlorophyll A; Darkness; Plant Leaves; Plants

1963
A NUCLEAR MAGNETIC RESONANCE STUDY OF HYDROGEN EXCHANGE AT METHINE POSITIONS IN CHLOROPHYLL A, CHLOROPHYLL B, CHLOROBIUM CHLOROPHYLL-660, AND BACTERIOCHLOROPHYLL.
    Biochemical and biophysical research communications, 1963, Sep-10, Volume: 13

    Topics: Bacteria; Bacteriochlorophylls; Biochemical Phenomena; Biochemistry; Chlorobium; Chlorophyll; Chlorophyll A; Hydrogen; Magnetic Resonance Spectroscopy; Research; Rhodospirillum

1963
Studies on the second Emerson effect in the Hill reaction in algal cells.
    Biophysical journal, 1961, Volume: 1

    This paper shows that the "second Emerson effect"1 exists not only in photosynthesis, but also in the quinone reduction (Hill reaction), in Chlorella pyrenoidosa and Anacystis nidulans. The peaks at 650 mmu, 600 mmu, 560 mmu, 520 mmu, and 480 mmu, observed in the action spectrum of this effect in the Hill reaction in Chorella, are attributable to chlorophyll b; the occurrence of an additional peak at 670 mmu, 620 mmu, and of two (or three) peaks in the blueviolet region suggests that (at least) one form of chlorophyll a contributes to it. In analogy to suggestions made previously in the interpretation of the Emerson effect in photosynthesis, these results are taken as indicating that excitation by light preferentially absorbed by one (or two) forms of chlorophyll a (Chl a 690 + 700), needs support by simultaneous absorption of light in another form of chlorophyll a (Chl a 670)-directly or via energy transfer from chlorophyll b-in order to produce the Hill reaction with its full quantum yield. In Anacystis, the participation of phycocyanin in the Emerson effect in the Hill reaction is revealed by the occurrence, in the action spectrum of this effect, of peaks at about 560 mmu, 610 mmu, and 640 mmu; a peak at 670 mmu, due to Chl a 670, also is present.

    Topics: Chlorophyll; Chlorophyll A; Chlorophyta; Energy Transfer; Eukaryota; Light; Photosynthesis; Phycocyanin; Quinones

1961
Two forms of chlorophyll a in vivo with distinct photochemical functions.
    Science (New York, N.Y.), 1960, Aug-05, Volume: 132, Issue:3423

    Action spectra of the Emerson effect in Chlorella and Navicula reveal peaks at 670 mut, in addition to those at 650 mu (Chlorella) and 630 mmicro (Navicula) attributable to chlorophylls b and c. Thus, excitation of chlorophyll a form "chlorophyll a 670" can supplement, in these algae, the excitation of the form "chlorophyll a 680-700." The effect of the auxiliary pigments in these algae may be mediated by energy transfer to "chlorophyll a 670."

    Topics: Chlorophyll; Chlorophyll A; Energy Transfer; Plants

1960