chlorophyll-a and thidiazuron

Chemical defoliation is an important part of cotton mechanical harvesting, which can effectively reduce the impurity content. Thidiazuron (TDZ) is the most used chemical defoliant on cotton. To better clarify the mechanism of TDZ promoting cotton leaf abscission, a greenhouse experiment was conducted on two cotton cultivars (CRI 12 and CRI 49) by using 100 mg L

Topics: Carbohydrates; Chlorophyll; Cotton Fiber; Defoliants, Chemical; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Ontology; Gossypium; Malondialdehyde; Microscopy, Electron, Scanning; Phenylurea Compounds; Photosynthesis; Plant Epidermis; Plant Leaves; Plant Stomata; Reactive Oxygen Species; RNA-Seq; Seedlings; Thiadiazoles

Different explants of fenugreek, T. foenum-graecum L. (Var. RMt-303), were compared for their callus induction and subsequent shoot regeneration capabilities on Murashige and Skoog media supplemented with different phytohormones in varying concentration. The highest percentage of callus induction frequency was observed in 1 ppm benzylaminopurine (BAP). Maximum shoots were induced on media supplemented with 0.5 ppm BAP using leaf and stem tissues as explants. However, root tissues showed only callusing with no subsequent shooting. Cotyledonary node responded better than hypocotyls in terms of shoot induction on media supplemented with thidiazuron (0.1 ppm). The callus was subjected to drought stress as simulated by reduced water potential of growth media due to addition of mannitol. Calli could withstand -2 MPa water potential till 30 days indicating that the drought stress tolerance mechanisms are functional in this variety. Chlorophyll a and b and total chlorophyll, proline and total phenolic contents, total peroxidase and catalase activities increased under stress conditions suggesting the tolerance of callus to drought stress. However, ascorbate peroxidase, guaiacol peroxidase activities were found to decrease slightly. Malondialdehyde and H2O2 contents were found to decrease while only a slight disturbance was found in membrane stability index. These results underline the mechanisms that are crucial for drought stress tolerance in fenugreek.

Topics: Adaptation, Physiological; Catalase; Chlorophyll; Culture Media; Dehydration; Droughts; Mannitol; Organoids; Oxidative Stress; Peroxidases; Phenols; Phenylurea Compounds; Plant Cells; Plant Leaves; Plant Proteins; Plant Shoots; Plants, Medicinal; Proline; Regeneration; Stress, Physiological; Thiadiazoles; Trigonella