chlorophyll-a and florfenicol

Although the ecological impacts of antibiotics have received attention worldwide, research on the toxicity of florfenicol is still limited. We conducted a metabolomic and proteomic study on wheat (Triticum aestivum L.) seedlings to reveal the toxicological mechanism of florfenicol. The growth of the wheat seedlings was found to be inhibited by florfenicol. Antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase), malondialdehyde content and membrane permeability increased with increasing florfenicol concentration. The contents of chlorophyll and chlorophyll synthesis precursor substances (Proto IX, Mg-proto IX and Pchlide), photosynthetic and respiration rates, and chlorophyll fluorescence parameters decreased, indicating that photosynthesis was inhibited. The ultrastructure of chloroplasts was destroyed, as evidenced by the blurred membrane surface, irregular grana arrangement, irregular thylakoid lamella structure, and increased plastoglobuli number. Proteome analysis revealed that up-regulated proteins were highly involved in protein refolding, translation, oxidation-reduction, tricarboxylic acid cycle (TCA cycle), reactive oxygen species metabolic process, cellular oxidant detoxification, and response to oxidative stress. The down-regulated proteins were mainly enriched in photosynthesis-related pathways. In the metabolome analysis, the content of most of the metabolites in wheat leaves, such as carbohydrates and amino acids increased significantly (p < 0.05). Combined pathway analysis showed that florfenicol stress stimulated the TCA cycle pathway and downregulated the photosynthesis pathway.

Topics: Antioxidants; Chlorophyll; Oxidative Stress; Proteomics; Seedlings; Superoxide Dismutase; Triticum

Florfenicol (FFC) is one of the most universally used antibiotics in aquaculture, which is substitute for chloramphenicol extensively, while the massive residues in aquatic environment were assumed to threaten the non-target organisms. Present research investigated the effects of florfenicol on growth, chlorophyll content, photosynthesis, and antioxidant ability of Isochrysis galbana. The results showed that FFC at 0.001-1 mg/L stimulated the growth of I. galbana and increased the content of chlorophyll. In addition, photosynthesis of I. galbana was inhibited and the photosynthetic parameters were uplifted with the increased exposure duration and FFC concentration. Furthermore, superoxide dismutase (SOD), catalase (CAT) activity significantly dropped at 0.01-20 mg/L FFC, while the contents of malondialdehyde (MDA), glutathione (GSH) and reactive oxygen species (ROS) increased after 72 h exposure, indicating that FFC at high concentrations caused a serious oxidative stress on algae. The simultaneous increase of ROS disrupted the equilibration between oxidants and antioxidant systems. Under the high concentration of FFC, the excessive of ROS was generated in algae which affected the membrane permeability and further decreased the cell biomass. Present study showed that acute exposure (72 h) at the environmental relevant concentration (0.01 mg/L) cannot induce the physiological dysfunction of the microalgae I. galbana, but the feeding concentration (20 mg/L) can. Additionally, this study hinted the possible negative impacts on ecosystems with the chronic exposure even at low FFC concentration or with the uncontrolled use of FFC.

Topics: Anti-Bacterial Agents; Antioxidants; Chlorophyll; Haptophyta; Oxidative Stress; Photosynthesis; Reactive Oxygen Species; Thiamphenicol; Water Pollutants, Chemical

Florfenicol (FF) and thiamphenicol (TAP) are two typical pharmaceuticals used widely as therapeutica antibiotic agents in aquaculture. However, little is known about the potential adverse effects of these two antibiotics on non-target organisms in the aquatic ecosystem. In this study we investigated the effects of FF and TAP on photosynthesis and the antioxidant system of the cyanobacteria Microcystis flos-aquae. Over a concentration range of 0.001-1μg/L, the results showed that both FF and TAP significantly increased the chlorophyll a content of M. flos-aquae, while the superoxide dismutase (SOD) activity, catalase (CAT) activity and the levels of malondialdehyde (MDA) changed slightly. In contrast, the chlorophyll a content of M. flos-aqua was significantly inhibited (p<0.01) at high concentrations (>1μg/L) of FF and TAP, reaching a 46% inhibition level at 50μg/L FF and 56% inhibition at 100μg/L TAP. At the same time, the activities of SOD and CAT along with MDA content also increased significantly (p<0.01), indicating that the high concentrations of both FF and TAP led to oxidative stress in the algae. In addition, the M. flos-aquae fluorescence parameters (Fv/Fm, Fv/Fo, alpha, ETRmax and Ik) increased with increasing concentration of both FF and TAP, which may be the result of the increasing photoprotection capacity.

Topics: Algal Proteins; Antioxidants; Catalase; Chlorophyll; Chlorophyll A; Environmental Exposure; Lipid Peroxidation; Lipid Peroxides; Malondialdehyde; Microcystis; Oxidative Stress; Photosynthesis; Superoxide Dismutase; Thiamphenicol; Water Pollutants, Chemical

Aquaculture facilities are a potential source of antibiotics to the aquatic ecosystems. The presence of these compounds in the environment may have deleterious effects on non-target aquatic organisms such as microalgae, which are often used as biological indicators of pollution. Therefore, the toxicity induced by chloramphenicol (CHL), florphenicol (FLO) and oxytetracycline (OTC), three antibiotics widely used in aquaculture, on the marine microalga Tetraselmis suecica was evaluated. Growth inhibition and physiological and biochemical parameters were analysed. All three antibiotics inhibited growth of T. suecica with 96 h IC50 values of 11.16, 9.03 and 17.25 mg L(-1) for CHL, FLO and OTC, respectively. After 24 h of exposure no effects on growth were observed and cell viability was also unaffected, whereas a decrease in esterase activity, related with cell vitality, was observed at the higher concentrations assayed. Photosynthesis related parameters such as chlorophyll a cellular content and autofluorescence were also altered after 24 h of antibiotics addition. It can be concluded that T. suecica was sensitive to the three antibiotics tested.

Topics: Anti-Bacterial Agents; Aquaculture; Cell Proliferation; Cell Survival; Chloramphenicol; Chlorophyll; Chlorophyll A; Microalgae; Oxytetracycline; Thiamphenicol; Toxicity Tests; Water Pollutants, Chemical

Effects of the phenicol antibiotic, florfenicol (0.5, 1.0, 2.0, 4.0, 8.0 and 16.0 mg/L), on marine diatom Skeletonema costatum were investigated in this study. Florfenicol was found to stimulate algal growth at concentrations of 0.5, 1.0 and 2.0 mg/L, and significantly inhibit algal growth at >2.0 mg/L. The highest inhibition rate was up to 86% at 16.0 mg/L and the IC(50) for 96 h growth was 5.043 mg/L. The chlorophyll a and effective quantum yield (ΔF/F(m)(')) were significantly inhibited at 6, 24 and 96 h when florfenicol concentrations were ≥4.0 mg/L. Intracellular reactive oxygen species (ROS) production was enhanced significantly over the control when florfenicol concentrations were ≥1.0 mg/L at 6 h with the dose-dependent trends possibly due to the inhibition of photosynthesis. Since the membrane is highly prone to ROS attack, overproduction of ROS may cause deteriorated integrity and permeability of the cell membrane. Consequently, intracellular pH was found to increase with the increases in dosage; cell size swelled significantly when alga was exposed to florfenicol concentrations up to 8.0 mg/L. These deteriorations finally led to the decrease of cell viability as indicated by both fluorescein diacetate (FDA) assay and propidium iodide (PI) staining, in which viability was shown to decrease significantly at higher doses (4.0, 8.0, 16.0 mg/L). It can be concluded that S. costatum was vulnerable to florfenicol.

Topics: Anti-Bacterial Agents; Cell Membrane Permeability; Cell Size; Cell Survival; Chlorophyll; Diatoms; Dose-Response Relationship, Drug; Hydrogen-Ion Concentration; Inhibitory Concentration 50; Oxidative Stress; Photosynthesis; Reactive Oxygen Species; Thiamphenicol