chlorogenic-acid and myricetin

chlorogenic-acid has been researched along with myricetin* in 2 studies

Other Studies

2 other study(ies) available for chlorogenic-acid and myricetin

Article	Year
Development of a fluorometric microplate antiadhesion assay using uropathogenic Escherichia coli and human uroepithelial cells. Journal of natural products, 2014, May-23, Volume: 77, Issue:5 A fluorometric microplate assay has been developed to determine Escherichia (E.) coli adhesion to uroepithelial cells (UEC). P-fimbriated E. coli were labeled with BacLight Green and preincubated 30 min with human urine or standard. Fluorescent-E. coli were added to UEC in mircoplates at a 400:1 ratio, incubated 1 h, and washed, and the fluorescence intensity was measured. Specific labeling and adherence were confirmed by flow cytometry. A myricetin (1) standard curve (0-30 μg/mL) was developed; the lower limit of detection was 0.1 μg/mL, and half-maximal inhibitory concentration was 0.88 μg/mL (intra- and interassay coefficients of variance were <10% and <15%, respectively). Vaccinium macrocarpon (cranberry) extracts, quercetin (2), and procyanidins B1 (3), B2 (4), and C1 (5) showed similar inhibition. Antiadhesion activity of urine samples from subjects (n = 12) consuming placebo or V. macrocarpon beverage determined using this assay was positively correlated (R(2) = 0.78; p < 0.01) with a radiolabeled-E. coli assay. Topics: Bacterial Adhesion; Biflavonoids; Catechin; Epithelial Cells; Flavonoids; Fruit; Humans; Molecular Structure; Plant Extracts; Proanthocyanidins; Quercetin; Urine; Uropathogenic Escherichia coli; Vaccinium macrocarpon	2014
Identification of phenolic constituents in Cichorium endivia var. crispum and var. latifolium salads by high-performance liquid chromatography with diode array detection and electrospray ioniziation tandem mass spectrometry. Journal of agricultural and food chemistry, 2012, Dec-12, Volume: 60, Issue:49 Chicory is a widely consumed vegetable and a source of phenolic compounds. Phenolic acid and flavonoid derivatives were identified in Cichorium endivia var. crispum and var. latifolium and fully characterized using complementary information from two different high-performance liquid chromatography detectors, diode array and mass spectrometer, in positive and negative modes. We describe about 40 phenolic compounds, some of which have never previously been reported in these plants, such as hydroxycinnamic acid derivatives (i.e., different mono- and dicaffeoylquinic acid isomers) and mono- and diglycosides of quercetin, kaempferol, and myricetin (differing also by the glycosylation site). These data provide a contribution to a more exhaustive identification of phenolic compounds in C. endivia vegetables. Topics: Chromatography, High Pressure Liquid; Cichorium intybus; Coumaric Acids; Flavonoids; Flavonols; Food Analysis; Isomerism; Kaempferols; Phenols; Quercetin; Quinic Acid; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry	2012

Article

Year

Development of a fluorometric microplate antiadhesion assay using uropathogenic Escherichia coli and human uroepithelial cells.

Journal of natural products, 2014, May-23, Volume: 77, Issue:5

A fluorometric microplate assay has been developed to determine Escherichia (E.) coli adhesion to uroepithelial cells (UEC). P-fimbriated E. coli were labeled with BacLight Green and preincubated 30 min with human urine or standard. Fluorescent-E. coli were added to UEC in mircoplates at a 400:1 ratio, incubated 1 h, and washed, and the fluorescence intensity was measured. Specific labeling and adherence were confirmed by flow cytometry. A myricetin (1) standard curve (0-30 μg/mL) was developed; the lower limit of detection was 0.1 μg/mL, and half-maximal inhibitory concentration was 0.88 μg/mL (intra- and interassay coefficients of variance were <10% and <15%, respectively). Vaccinium macrocarpon (cranberry) extracts, quercetin (2), and procyanidins B1 (3), B2 (4), and C1 (5) showed similar inhibition. Antiadhesion activity of urine samples from subjects (n = 12) consuming placebo or V. macrocarpon beverage determined using this assay was positively correlated (R(2) = 0.78; p < 0.01) with a radiolabeled-E. coli assay.

Topics: Bacterial Adhesion; Biflavonoids; Catechin; Epithelial Cells; Flavonoids; Fruit; Humans; Molecular Structure; Plant Extracts; Proanthocyanidins; Quercetin; Urine; Uropathogenic Escherichia coli; Vaccinium macrocarpon

2014

Identification of phenolic constituents in Cichorium endivia var. crispum and var. latifolium salads by high-performance liquid chromatography with diode array detection and electrospray ioniziation tandem mass spectrometry.

Journal of agricultural and food chemistry, 2012, Dec-12, Volume: 60, Issue:49

Chicory is a widely consumed vegetable and a source of phenolic compounds. Phenolic acid and flavonoid derivatives were identified in Cichorium endivia var. crispum and var. latifolium and fully characterized using complementary information from two different high-performance liquid chromatography detectors, diode array and mass spectrometer, in positive and negative modes. We describe about 40 phenolic compounds, some of which have never previously been reported in these plants, such as hydroxycinnamic acid derivatives (i.e., different mono- and dicaffeoylquinic acid isomers) and mono- and diglycosides of quercetin, kaempferol, and myricetin (differing also by the glycosylation site). These data provide a contribution to a more exhaustive identification of phenolic compounds in C. endivia vegetables.

Topics: Chromatography, High Pressure Liquid; Cichorium intybus; Coumaric Acids; Flavonoids; Flavonols; Food Analysis; Isomerism; Kaempferols; Phenols; Quercetin; Quinic Acid; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry

2012