chlorin-p6 and chlorin

The use of photoactivated disinfection has had a significant medical and technological effect in bacterial inactivation, as an alternative to conventional antimicrobial methods. The main goal of this study was to investigate the effect of photoactivated disinfection on Streptococcus mutans, when Radachlorin(®) was used as a photosensitizer.. Streptococcus mutans samples of two different initial concentrations were treated with Radachlorin(®) gel (0.1%), irradiated by the light of a He-Ne laser (633nm), with energy density of 6J/cm(2), and cell viability was evaluated after culturing.. It was observed that the combination of Radachlorin(®) and laser was more effective than Radachlorin(®) or laser alone (p<0.05), in reduction of S. mutans and Radachlorin(®) was cytotoxic, in the dark, only for the lower concentration of bacteria. Lower concentration of S. mutans resulted in higher amount of killing, in the case of using Radachlorin(®) with or without laser.. The photoactivation of Radachlorin(®) using a He-Ne laser could inactivate S. mutans to a significant level. In addition Radachlorin(®) might be cytotoxic in the dark, for the lower concentration of bacteria.

Topics: Anti-Bacterial Agents; Bacterial Load; Chlorophyllides; Darkness; Disinfection; Drug Combinations; Humans; Lasers, Gas; Materials Testing; Microbial Viability; Photosensitizing Agents; Porphyrins; Radiation Dosage; Streptococcus mutans

The binding of serum albumin and lipoprotein with chlorin p(6) and purpurin 18, two structurally related chlorins, has been studied to understand the role for these proteins as endogenous carriers for these drugs. As a drug carrier a protein may aid in selective delivery of a drug to a tumor region. Binding with serum albumin may result in accumulation of the drug in the stroma of the tumor cell and lead to a reduction of cellular uptake of photosensitizers. However, it is possible that this factor may not be a problem for cellular uptake of chlorin p(6) and purpurin 18 by the tumor tissues, since it binds more efficiently with low-density lipoprotein when it become more lipophilic, indicating that the principal carriers for these molecules are lipoproteins. Since the tumor tissues contain numerous lipoprotein receptors, chlorin p(6) and purpurin 18 could be internalized more efficiently in tumor cells.

Topics: Drug Carriers; Hydrophobic and Hydrophilic Interactions; Lipoproteins; Photochemotherapy; Photosensitizing Agents; Plasma; Porphyrins; Protein Binding; Serum Albumin