cgp-37849 and pyrrolidine-2-4-dicarboxylic-acid

cgp-37849 has been researched along with pyrrolidine-2-4-dicarboxylic-acid* in 1 studies

Other Studies

1 other study(ies) available for cgp-37849 and pyrrolidine-2-4-dicarboxylic-acid

Article	Year
Prolonged enhancement of AP-1 DNA binding by blockade of glutamate uptake in cultured neurons. Neuroreport, 1999, Jun-23, Volume: 10, Issue:9 Prolonged blockade of glutamate reuptake by the specific inhibitor of glutamate transporters, L-transpyrrolidine-2,4-dicarboxylate (PDC), produces a dramatic decrease in NMDA-induced neurotoxicity in cerebellar granule cell cultures, and is accompanied by a down-regulation of NMDA receptors. We now report that cultured cerebellar granule cells treated with 100 microM PDC for 1, 2, 4, 8, 16 and 24h, respectively, show increased AP-1 DNA-binding activity as measured by electrophoretic mobility shift assay. This effect was blocked by the NMDA receptor antagonist, CGP 37849, indicative of a pivotal role of NMDA receptors in the PDC-evoked enhancement of AP-1 DNA-binding. Our results suggest that AP-1 may be involved in the transcriptional regulation of neuronal adaptation initiated by prolonged inhibition of glutamate reuptake. Topics: 2-Amino-5-phosphonovalerate; Animals; Biological Transport; Cells, Cultured; Cerebellum; Dicarboxylic Acids; Dimerization; Dizocilpine Maleate; DNA-Binding Proteins; Excitatory Amino Acid Antagonists; Glutamic Acid; Neurons; Neurotoxins; Neurotransmitter Uptake Inhibitors; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; Pyrrolidines; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; Transcription Factor AP-1	1999

Article

Year

Prolonged enhancement of AP-1 DNA binding by blockade of glutamate uptake in cultured neurons.

Neuroreport, 1999, Jun-23, Volume: 10, Issue:9

Prolonged blockade of glutamate reuptake by the specific inhibitor of glutamate transporters, L-transpyrrolidine-2,4-dicarboxylate (PDC), produces a dramatic decrease in NMDA-induced neurotoxicity in cerebellar granule cell cultures, and is accompanied by a down-regulation of NMDA receptors. We now report that cultured cerebellar granule cells treated with 100 microM PDC for 1, 2, 4, 8, 16 and 24h, respectively, show increased AP-1 DNA-binding activity as measured by electrophoretic mobility shift assay. This effect was blocked by the NMDA receptor antagonist, CGP 37849, indicative of a pivotal role of NMDA receptors in the PDC-evoked enhancement of AP-1 DNA-binding. Our results suggest that AP-1 may be involved in the transcriptional regulation of neuronal adaptation initiated by prolonged inhibition of glutamate reuptake.

Topics: 2-Amino-5-phosphonovalerate; Animals; Biological Transport; Cells, Cultured; Cerebellum; Dicarboxylic Acids; Dimerization; Dizocilpine Maleate; DNA-Binding Proteins; Excitatory Amino Acid Antagonists; Glutamic Acid; Neurons; Neurotoxins; Neurotransmitter Uptake Inhibitors; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; Pyrrolidines; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; Transcription Factor AP-1

1999