cerulenin and decanoic-acid

cerulenin has been researched along with decanoic-acid* in 2 studies

Other Studies

2 other study(ies) available for cerulenin and decanoic-acid

Article	Year
Specialized fatty acid synthesis in African trypanosomes: myristate for GPI anchors. Science (New York, N.Y.), 2000, Apr-07, Volume: 288, Issue:5463 African trypanosomes, the cause of sleeping sickness, need massive amounts of myristate to remodel glycosyl phosphatidylinositol (GPI) anchors on their surface glycoproteins. However, it has been believed that the parasite is unable to synthesize any fatty acids, and myristate is not abundant in the hosts' bloodstreams. Thus, it has been unclear how trypanosomes meet their myristate requirement. Here we found that they could indeed synthesize fatty acids. The synthetic pathway was unique in that the major product, myristate, was preferentially incorporated into GPIs and not into other lipids. The antibiotic thiolactomycin inhibited myristate synthesis and killed the parasite, making this pathway a potential chemotherapeutic target. Topics: Acyl Coenzyme A; Animals; Anti-Bacterial Agents; Caprylates; Cell-Free System; Cerulenin; Decanoic Acids; Fatty Acid Synthases; Fatty Acids; Glycosylphosphatidylinositols; Laurates; Myristates; Subcellular Fractions; Thiophenes; Trypanosoma brucei brucei	2000
Evidence for the synthesis of the multi-positional isomers of monounsaturated fatty acid in Methylococcus capsusatus by the anaerobic pathway. FEMS microbiology letters, 1989, Volume: 58 The biosynthesis of the positional isomers of the monounsaturated fatty acids of Methylococcus capsulatus (Bath) has been investigated by studying the incorporation of [2-14C]malonyl CoA into long-chain fatty acids in vitro. The major unsaturated products were delta 9 16 : 1 and delta 11 18 : 1; however, delta 8, delta 10, and delta 11, 16 : 1, as well as, delta 10, delta 12 and delta 13 18 : 1 were also synthesized. The exclusion of O2 from the reaction vessel did not affect the synthesis of unsaturated fatty acids or the double bonds positions. Cerulenin inhibited the synthesis of unsaturated fatty acid more than saturated fatty acid. The use of both [1-14C] octanoate and [1-14C] decanote as substrate resulted in the synthesis of long-chain fatty acids, however, unsaturates were only synthesized from octanoate. These results imply that the unique positional isomers of M. capsulatus are not synthesized by an aerobic mechanism. Topics: Anaerobiosis; Antifungal Agents; Caprylates; Cerulenin; Decanoic Acids; Fatty Acids; Fatty Acids, Monounsaturated; Fatty Acids, Unsaturated; Isomerism; Malonyl Coenzyme A; Methylococcaceae; Oxygen	1989

Article

Year

Specialized fatty acid synthesis in African trypanosomes: myristate for GPI anchors.

Science (New York, N.Y.), 2000, Apr-07, Volume: 288, Issue:5463

African trypanosomes, the cause of sleeping sickness, need massive amounts of myristate to remodel glycosyl phosphatidylinositol (GPI) anchors on their surface glycoproteins. However, it has been believed that the parasite is unable to synthesize any fatty acids, and myristate is not abundant in the hosts' bloodstreams. Thus, it has been unclear how trypanosomes meet their myristate requirement. Here we found that they could indeed synthesize fatty acids. The synthetic pathway was unique in that the major product, myristate, was preferentially incorporated into GPIs and not into other lipids. The antibiotic thiolactomycin inhibited myristate synthesis and killed the parasite, making this pathway a potential chemotherapeutic target.

Topics: Acyl Coenzyme A; Animals; Anti-Bacterial Agents; Caprylates; Cell-Free System; Cerulenin; Decanoic Acids; Fatty Acid Synthases; Fatty Acids; Glycosylphosphatidylinositols; Laurates; Myristates; Subcellular Fractions; Thiophenes; Trypanosoma brucei brucei

2000

Evidence for the synthesis of the multi-positional isomers of monounsaturated fatty acid in Methylococcus capsusatus by the anaerobic pathway.

FEMS microbiology letters, 1989, Volume: 58

The biosynthesis of the positional isomers of the monounsaturated fatty acids of Methylococcus capsulatus (Bath) has been investigated by studying the incorporation of [2-14C]malonyl CoA into long-chain fatty acids in vitro. The major unsaturated products were delta 9 16 : 1 and delta 11 18 : 1; however, delta 8, delta 10, and delta 11, 16 : 1, as well as, delta 10, delta 12 and delta 13 18 : 1 were also synthesized. The exclusion of O2 from the reaction vessel did not affect the synthesis of unsaturated fatty acids or the double bonds positions. Cerulenin inhibited the synthesis of unsaturated fatty acid more than saturated fatty acid. The use of both [1-14C] octanoate and [1-14C] decanote as substrate resulted in the synthesis of long-chain fatty acids, however, unsaturates were only synthesized from octanoate. These results imply that the unique positional isomers of M. capsulatus are not synthesized by an aerobic mechanism.

Topics: Anaerobiosis; Antifungal Agents; Caprylates; Cerulenin; Decanoic Acids; Fatty Acids; Fatty Acids, Monounsaturated; Fatty Acids, Unsaturated; Isomerism; Malonyl Coenzyme A; Methylococcaceae; Oxygen

1989