ceragenin-csa-13 and dodecylamine

To determine how the microbicide ceragenin-13 (CSA-13) kills Bacillus subtilis spores prepared on growth or sporulation media, and these spores' properties.. Spores made on Luria broth (LB) growth or double-strength Schaeffer's-glucose (2xSG) sporulation plates found that spores made on LB plates have coat defects as evidenced by their lower hypochlorite resistance, faster germination with dodecylamine and slower germination with Ca. CSA-13 kills bacterial spores, but only at high concentrations and temperatures, and is preceded by CaDPA release.. CSA-13 is not a direct sporicide as reported previously, but most likely germinates spores via activation of spores' CaDPA channel, albeit inefficiently, and then killing the germinated spores.

Topics: Amines; Anti-Infective Agents; Bacillus subtilis; Culture Media; Picolinic Acids; Spores, Bacterial; Steroids