cephalomannine and tetracarboxyphenylporphine

cephalomannine has been researched along with tetracarboxyphenylporphine* in 1 studies

Other Studies

1 other study(ies) available for cephalomannine and tetracarboxyphenylporphine

Article	Year
A novel bioassay for screening and quantification of taxanes. Chemical communications (Cambridge, England), 2003, May-21, Issue:10 We report a novel method for screening and quantifying tubulin-binding antimitotic agents that promote microtubule formation. The method is based on the shift in the peak of the fluorescence emission difference spectrum of tubulin complexed to metal free tetrakis(4-carboxyphenyl)porphyrin (TCPP) in the presence of antimitotic agents. Taxol (paclitaxel), an anti-tumor drug approved for the treatment of a variety of cancers, caused the appearance of a new fluorescence peak at 645 nm at concentrations as low as 125 nM, the intensity of which was a function of the paclitaxel concentration. Cephalomannine caused the induction of a new fluorescence peak at 651 nm only above 1 muM. Baccatin did not induce the appearance of any new peak within detectable operating measurement conditions. These observations are in accordance with the biological activities/cytotoxicities of these compounds. Accordingly, it is proposed that the new method can be used for high throughput screening of antimitotic compounds. Topics: Antineoplastic Agents, Phytogenic; Biological Assay; Fluorescence; Molecular Structure; Paclitaxel; Porphyrins; Taxoids; Tubulin	2003

Article

Year

A novel bioassay for screening and quantification of taxanes.

Chemical communications (Cambridge, England), 2003, May-21, Issue:10

We report a novel method for screening and quantifying tubulin-binding antimitotic agents that promote microtubule formation. The method is based on the shift in the peak of the fluorescence emission difference spectrum of tubulin complexed to metal free tetrakis(4-carboxyphenyl)porphyrin (TCPP) in the presence of antimitotic agents. Taxol (paclitaxel), an anti-tumor drug approved for the treatment of a variety of cancers, caused the appearance of a new fluorescence peak at 645 nm at concentrations as low as 125 nM, the intensity of which was a function of the paclitaxel concentration. Cephalomannine caused the induction of a new fluorescence peak at 651 nm only above 1 muM. Baccatin did not induce the appearance of any new peak within detectable operating measurement conditions. These observations are in accordance with the biological activities/cytotoxicities of these compounds. Accordingly, it is proposed that the new method can be used for high throughput screening of antimitotic compounds.

Topics: Antineoplastic Agents, Phytogenic; Biological Assay; Fluorescence; Molecular Structure; Paclitaxel; Porphyrins; Taxoids; Tubulin

2003