cembrene-a and methyl-jasmonate

In plants, the ABC transporter PDR (pleiotropic drug resistance) subfamily is composed of approximately 15 genes, few of which have been analyzed. We have identified NtPDR3, a Nicotiana tabacum PDR gene belonging to a cluster for which no functional data was previously available. NtPDR3 was found to be induced in suspension cells treated with methyl jasmonate, salicylic acid, 1-naphthalene acetic acid, or cembrene, a macrocyclic diterpene. In agreement with the identification of a putative iron deficiency element in the NtPDR3 transcription promoter region, we found that iron deficiency in the culture medium induced NtPDR3 expression, thus suggesting a new function of the PDR transporter family.

Topics: Acetates; ATP-Binding Cassette Transporters; Base Sequence; Biological Transport; Cells, Cultured; Chelating Agents; Cloning, Molecular; Consensus Sequence; Culture Media; Cyclopentanes; Diterpenes; DNA, Complementary; Egtazic Acid; Gene Expression Regulation, Plant; Genes, Plant; Genetic Vectors; Iron Deficiencies; Molecular Sequence Data; Naphthaleneacetic Acids; Nicotiana; Oxylipins; Phylogeny; Plant Growth Regulators; Plant Proteins; Promoter Regions, Genetic; Salicylic Acid; Transcription, Genetic