cellulase and sodium-sulfite

Pretreatment is important to overcome the structural recalcitrance of reed (a viable energy grass) to produce fermentable sugar. Herein, the study reported the pretreatment of reed using different alkali chemicals (sodium hydroxide/anthraquinone, sodium hydroxide/sodium sulfite, sodium hydroxide/sodium sulfide, ammonia/hydrogen peroxide, triethanolamine, and ammonia/sodium sulfite). The comparative study showed that the pretreatment using ammonia and sodium sulfite (NS) performed the best among them. The NS pretreatment of reed was further optimized using the Response Surface Methodology (RSM). The results showed that about 90.36% lignin was removed when reed was pretreated with 10 wt% of ammonia and 10% of sodium sulfite at 172 °C for 20 min. The excellent lignin removal performance was attributable to the synergistic effects between ammonia and sodium sulfite. The NS pretreated reed achieved 85.6% of enzymatic hydrolysis efficiency and 64.83% of total sugar yield.

Topics: Ammonia; Cellulase; Hydrolysis; Lignin; Sodium Hydroxide; Sugars

The effects of sodium sulfite pretreatment on the delignification rate, cellulose content, enzymatic hydrolysis efficiency, and glucose yield of corncob residues (CCR) were investigated. The optimum pretreatment conditions were as follows: 12% sodium sulfite, with a pH value of 7, a temperature of 160 °C, and a holding time of 20 min. Under the optimal conditions, the cellulose content in the pretreated residue was 85.17%, and sodium lignosulfonate with a sulfonation degree of 0.677 mmol/g was obtained in the waste liquids. A delignification rate of 77.45% was also achieved after the pretreatment. Enzymatic hydrolysis of pretreated CCR was carried out with cellulase (5 FPU/g substrate) and β-glucosidase (10 IU/g substrate) for 48 h. The untreated CCR were hydrolyzed using cellulase (20 FPU/g substrate) and β-glucosidase (10 IU/g substrate) for 48 h. The comparison results showed that sodium sulfite pretreatment improved the enzymatic hydrolysis efficiency and glucose yield, which increased by 28.80% and 20.10%, respectively. These results indicated that despite the application of low cellulase dosage, high enzymatic hydrolysis efficiency substrate could be produced, and the sodium lignosulfonate which can be used for oilfields and concrete additives was obtained from the sodium sulfite-pretreated CCR.

Topics: beta-Glucosidase; Biotechnology; Cellulase; Glucose; Hydrolysis; Lignin; Sulfites; Temperature; Zea mays

In this work, mixed hardwoods were pretreated by sodium sulfite-formaldehyde (SF). The effects of SF pretreatment on the chemical compositions and enzymatic hydrolysis of mixed hardwoods were investigated. SF pretreatment temperature had a significant effect on pulp yield and delignification, resulting in an increased efficiency of enzymatic hydrolysis. After 96 h of enzymatic hydrolysis at the cellulase loading of 40 FPU/g substrate, the yields of glucan and xylan on the basis of original wood were 37% and 11% for the pulp produced with 12% sulfite charge at 170 °C for 2 h. The total sugar recovery based on the sugar in original wood was 74%. These results indicate that sulfite-formaldehyde cooking is of great potential to be a pretreatment method for a greenfield mill to produce fuel ethanol from hardwood.

Topics: Carbohydrate Metabolism; Cellulase; Formaldehyde; Glucans; Hydrogen-Ion Concentration; Hydrolysis; Lignin; Sulfites; Temperature; Time Factors; Wood

Agave americana is one of commonly grown agave species but currently less valuable because its large flower stalk cannot be used for producing alcoholic beverage. In the present study, the stalk was pretreated with dilute acid (DA), sulfite (SPORL), and sodium hydroxide (NaOH) to preliminarily assess its potential as feedstock for bioethanol production. The changes of cell wall components during the pretreatments, enzymatic digestibility of the pretreated stalks, and the adsorption of cellulases on the substrates were investigated. Results indicated that the pretreatments significantly improved the enzymatic digestibility of the agave stalk. SPORL pretreatment gave higher substrate and sugar yields, while NaOH pretreated stalk had better digestibility under the investigated conditions. The better hydrolysability of NaOH-pretreated stalk was attributed to low lignin and hemicellulose content and high affinity to cellulases.

Topics: Agave; Carbohydrate Metabolism; Cellulase; Cellulose; Hydrolysis; Sodium Hydroxide; Solid Waste; Sulfites; Sulfuric Acids; Time Factors; Xylans