cellulase and perlolyrine

cellulase has been researched along with perlolyrine* in 2 studies

Other Studies

2 other study(ies) available for cellulase and perlolyrine

Article	Year
Cellulolytic enzymes of rumen anaerobic fungi Orpinomyces joyonii and Caecomyces communis. Research in microbiology, 1998, Volume: 149, Issue:6 The rumen anaerobic fungi Orpinomyces joyonii A4 and Caecomyces communis JB1 were grown on microcrystalline cellulose (MC) and alfalfa hay. The cellular distribution of cellulases produced by these organisms was monitored. Fungal cultures were separated into extracellular, intracellular and cell wall fractions and assayed for endoglucanase (EG) and beta-glucosidase activity. In both fungal isolates, EG activity was the highest in the extracellular fraction regardless of the substrate used. The beta-glucosidase activity produced by O. joyonii was mainly found in the cell wall fraction. On the contrary, the same enzyme activity in C. communis predominated in the extracellular fraction. The polycentric isolate A4 more efficiently utilized both substrates, produced more short chain fatty acids (up to 31 mmol/l) and showed higher total levels of EG (2744 nmol glucose/h/ml) than the monocentric strain JB1. On the other hand, beta-glucosidase (9033 nmol glucose/h/ml) activity was the highest in cultures of C. communis grown on cellulose. In cultures of O. joyonii grown on MC, the production of yellow affinity substance (YAS) with similar properties compared with yellow substance from Clostridium thermocellum was observed. This compound increased the adsorption of fungal cellulases to MC the temperature and pH range tested. Topics: Adsorption; Anaerobiosis; Animals; beta-Glucosidase; Carbolines; Cellulase; Cellulose; Furans; Medicago sativa; Neocallimastigales; Rumen	1998
The effect of yellow affinity substance on cellulases of Ruminococcus flavefaciens. Letters in applied microbiology, 1997, Volume: 25, Issue:3 Cellulolytic cultures of Ruminococcus flavefaciens produced a yellow affinity substance (YAS) with a strong affinity to microcrystalline cellulose (MC). YAS was bound to MC in the range of pH from 5 to 8 and at temperatures from 10 degrees C to 60 degrees C. The positive effect of YAS on adsorption of ruminococcal cellulases was demonstrated by comparing the adsorption behaviour of endoglucanases and cellobiohydrolases onto MC and YAS-treated MC. HPLC chromatography proved the presence of two yellow compounds with affinity to cellulose as well as to ruminococcal cellulases. Both YAS compounds were sensitive to oxidation. The observed YAS properties showed a close relation to YS of Clostridium thermocellum. Topics: Adsorption; Animals; Bacteria, Anaerobic; Carbolines; Cellulase; Cellulose; Cellulose 1,4-beta-Cellobiosidase; Chromatography, High Pressure Liquid; Clostridium; Furans; In Vitro Techniques; Rumen	1997

Article

Year

Cellulolytic enzymes of rumen anaerobic fungi Orpinomyces joyonii and Caecomyces communis.

Research in microbiology, 1998, Volume: 149, Issue:6

The rumen anaerobic fungi Orpinomyces joyonii A4 and Caecomyces communis JB1 were grown on microcrystalline cellulose (MC) and alfalfa hay. The cellular distribution of cellulases produced by these organisms was monitored. Fungal cultures were separated into extracellular, intracellular and cell wall fractions and assayed for endoglucanase (EG) and beta-glucosidase activity. In both fungal isolates, EG activity was the highest in the extracellular fraction regardless of the substrate used. The beta-glucosidase activity produced by O. joyonii was mainly found in the cell wall fraction. On the contrary, the same enzyme activity in C. communis predominated in the extracellular fraction. The polycentric isolate A4 more efficiently utilized both substrates, produced more short chain fatty acids (up to 31 mmol/l) and showed higher total levels of EG (2744 nmol glucose/h/ml) than the monocentric strain JB1. On the other hand, beta-glucosidase (9033 nmol glucose/h/ml) activity was the highest in cultures of C. communis grown on cellulose. In cultures of O. joyonii grown on MC, the production of yellow affinity substance (YAS) with similar properties compared with yellow substance from Clostridium thermocellum was observed. This compound increased the adsorption of fungal cellulases to MC the temperature and pH range tested.

Topics: Adsorption; Anaerobiosis; Animals; beta-Glucosidase; Carbolines; Cellulase; Cellulose; Furans; Medicago sativa; Neocallimastigales; Rumen

1998

The effect of yellow affinity substance on cellulases of Ruminococcus flavefaciens.

Letters in applied microbiology, 1997, Volume: 25, Issue:3

Cellulolytic cultures of Ruminococcus flavefaciens produced a yellow affinity substance (YAS) with a strong affinity to microcrystalline cellulose (MC). YAS was bound to MC in the range of pH from 5 to 8 and at temperatures from 10 degrees C to 60 degrees C. The positive effect of YAS on adsorption of ruminococcal cellulases was demonstrated by comparing the adsorption behaviour of endoglucanases and cellobiohydrolases onto MC and YAS-treated MC. HPLC chromatography proved the presence of two yellow compounds with affinity to cellulose as well as to ruminococcal cellulases. Both YAS compounds were sensitive to oxidation. The observed YAS properties showed a close relation to YS of Clostridium thermocellum.

Topics: Adsorption; Animals; Bacteria, Anaerobic; Carbolines; Cellulase; Cellulose; Cellulose 1,4-beta-Cellobiosidase; Chromatography, High Pressure Liquid; Clostridium; Furans; In Vitro Techniques; Rumen

1997