cellulase and diphenyl

An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized. Cellulase was used for the degradation of AAP. The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM). The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2. Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%. The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid. The molecular weight of the degradation product was 5.94 × 10

Topics: Antioxidants; Auricularia; Biphenyl Compounds; Cellulase; Chromatography, High Pressure Liquid; Fucose; Fungal Polysaccharides; Galactose; Glucose; Glucuronic Acid; Hydrolysis; Hydroxyl Radical; Lactones; Magnetic Resonance Spectroscopy; Mannose; Molecular Weight; Monosaccharides; Rhamnose; Superoxides; Xylose