cellulase and 5-((2-aminoethyl)amino)naphthalene-1-sulfonic-acid

cellulase has been researched along with 5-((2-aminoethyl)amino)naphthalene-1-sulfonic-acid* in 1 studies

Other Studies

1 other study(ies) available for cellulase and 5-((2-aminoethyl)amino)naphthalene-1-sulfonic-acid

Article	Year
Chemoenzymatic synthesis of a bifunctionalized cellohexaoside as a specific substrate for the sensitive assay of cellulase by fluorescence quenching. Chemistry (Weinheim an der Bergstrasse, Germany), 2002, Mar-15, Volume: 8, Issue:6 A new bifunctionalized cellohexaose derivative was synthesized as a specific substrate for continuous assay of cellulases by resonance energy transfer. This cellohexaoside has a naphthalene moiety (EDANS) as a fluorescent energy donor at the reducing end and a 4-(4'-dimethylaminobenzeneazo)-benzene derivative as an acceptor chromophore at the non-reducing end. The key steps for the preparation of the target molecule involved transglycosylation reactions of cellobiosyl and cellotetraosyl fluoride donors onto cellobiosyl acceptors catalysed by the E197A mutant of cellulase Cel7B from Humicola insolens. Upon digestion with various cellulases, the energy transfer was disrupted and an increase of fluorescence was observed. Topics: Cellulase; Energy Transfer; Fluorometry; Indicators and Reagents; Naphthalenesulfonates; Oligosaccharides; Sensitivity and Specificity; Substrate Specificity	2002

Article

Year

Chemoenzymatic synthesis of a bifunctionalized cellohexaoside as a specific substrate for the sensitive assay of cellulase by fluorescence quenching.

Chemistry (Weinheim an der Bergstrasse, Germany), 2002, Mar-15, Volume: 8, Issue:6

A new bifunctionalized cellohexaose derivative was synthesized as a specific substrate for continuous assay of cellulases by resonance energy transfer. This cellohexaoside has a naphthalene moiety (EDANS) as a fluorescent energy donor at the reducing end and a 4-(4'-dimethylaminobenzeneazo)-benzene derivative as an acceptor chromophore at the non-reducing end. The key steps for the preparation of the target molecule involved transglycosylation reactions of cellobiosyl and cellotetraosyl fluoride donors onto cellobiosyl acceptors catalysed by the E197A mutant of cellulase Cel7B from Humicola insolens. Upon digestion with various cellulases, the energy transfer was disrupted and an increase of fluorescence was observed.

Topics: Cellulase; Energy Transfer; Fluorometry; Indicators and Reagents; Naphthalenesulfonates; Oligosaccharides; Sensitivity and Specificity; Substrate Specificity

2002