cellulase and 4-toluenesulfonic-acid

Modificated lignins can affect enzymatic hydrolysis efficiency (EHE) because of changing physicochemical properties of lignin. In this study, carboxylated and quaternized lignin (CQL) and hydroxymethylated lignin (HML) were prepared to explore the effect of lignin modification on cellulase adsorption and EHE of p-toluenesulfonic acid treated corn stover (PCS). The results showed that CQL enhanced EHE of PCS due to the higher β-glucosidase (β-GL) activity, resulting from the formation of CQL-β-GL complexes with a lower binding free energy and the improvement of β-GL conformation made by the binding of CQL and β-GL. However, the drop in EHE due to the addition of HML was consequent on β-GL deactivation that was because the binding site of HML and β-GL overlapped with the carbohydrate binding domain of β-GL, causing the decrease in β-GL activity compared with CQL. This study would help deeply elucidate the effect of modified lignins on EHE and cellulase adsorption.

Topics: Adsorption; Benzenesulfonates; Cellulase; Hydrolysis; Lignin

Here we established a new approach for improving the cellulose nanofibrillation of high ash content waste wheat straw (WWS). The results were comprehensively elucidated from the ash removal, delignification, mechanical fibrillation and endoglucanase post-treatment. When water dosage was increased from 50 to 500 times of the WWS weight, the ash content gradually decreased during prewashing process, which facilitated lignin solubilization in subsequent p-toluenesulfonic acid (p-TsOH) hydrolysis. Approximately 80% of lignin in prewashed WWS could be dissolved during acid hydrolysis to result in a relatively higher crystallinity of 59.1%. Compared with the lignocellulosic nanofibrils (LCNF) directly obtained using acid hydrolysis and disk grinding, prewashing-assisted acid hydrolyzed WWS was fibrillated into LCNF with smaller height of 57.0 nm. Mild endoglucanase post-treatment could further produce less entangled LCNF with thinner diameters. In short, this study presented a promising and green pathway to achieve an efficient utilization of agricultural residue wastes to cellulose nanomaterials.

Topics: Benzenesulfonates; Cellulase; Cellulose; Hydrolysis; Lignin; Triticum