cellulase and 3-5-dinitrosalicylic-acid

cellulase has been researched along with 3-5-dinitrosalicylic-acid* in 2 studies

Other Studies

2 other study(ies) available for cellulase and 3-5-dinitrosalicylic-acid

Article	Year
Effective microwell plate-based screening method for microbes producing cellulase and xylanase and its application. Journal of microbiology and biotechnology, 2014, Nov-28, Volume: 24, Issue:11 Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a 200 μl total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at 100°C for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass. Topics: Bacteria; Cellulase; Colorimetry; Endo-1,4-beta Xylanases; Enzyme Assays; Fungi; Salicylates	2014
Suggested improvements to the standard filter paper assay used to measure cellulase activity. Biotechnology and bioengineering, 2003, Jun-20, Volume: 82, Issue:6 Two suggestions can be found in the literature to improve the reproducibility of the Mandels' filter paper assay: add supplemental cellobiase and increase the boiling time for color development. Here we provide data that strongly supports adding supplemental cellobiase. Adding supplemental cellobiase increased assay response by 56%. Cellulases from different sources have different cellobiase activities, which would cause significant variation in the assay response. There is no need for additional boiling time-5 minutes is sufficient. For maximum reproducibility, it is essential that the water bath vigorously boil so that temperature excursions are minimized. Topics: beta-Glucosidase; Biosensing Techniques; Cellulase; Colorimetry; Enzyme Activation; Filtration; Hot Temperature; Quality Control; Salicylates; Sensitivity and Specificity; Substrate Specificity	2003

Article

Year

Effective microwell plate-based screening method for microbes producing cellulase and xylanase and its application.

Journal of microbiology and biotechnology, 2014, Nov-28, Volume: 24, Issue:11

Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a 200 μl total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at 100°C for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.

Topics: Bacteria; Cellulase; Colorimetry; Endo-1,4-beta Xylanases; Enzyme Assays; Fungi; Salicylates

2014

Biotechnology and bioengineering, 2003, Jun-20, Volume: 82, Issue:6

Two suggestions can be found in the literature to improve the reproducibility of the Mandels' filter paper assay: add supplemental cellobiase and increase the boiling time for color development. Here we provide data that strongly supports adding supplemental cellobiase. Adding supplemental cellobiase increased assay response by 56%. Cellulases from different sources have different cellobiase activities, which would cause significant variation in the assay response. There is no need for additional boiling time-5 minutes is sufficient. For maximum reproducibility, it is essential that the water bath vigorously boil so that temperature excursions are minimized.

Topics: beta-Glucosidase; Biosensing Techniques; Cellulase; Colorimetry; Enzyme Activation; Filtration; Hot Temperature; Quality Control; Salicylates; Sensitivity and Specificity; Substrate Specificity

2003