cellobiosyl-fluoride and acetonitrile

cellobiosyl-fluoride has been researched along with acetonitrile* in 1 studies

Other Studies

1 other study(ies) available for cellobiosyl-fluoride and acetonitrile

Article	Year
Assembly of synthetic cellulose I. Proceedings of the National Academy of Sciences of the United States of America, 1994, Aug-02, Volume: 91, Issue:16 Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of beta-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph. Topics: Acetates; Acetonitriles; Buffers; Carbohydrate Sequence; Cellobiose; Cellulase; Cellulose; Cellulose 1,4-beta-Cellobiosidase; Electrons; Glycoside Hydrolases; Gold Colloid; Microscopy, Electron; Molecular Sequence Data; Scattering, Radiation; Staining and Labeling; Trichoderma	1994

Article

Year

Proceedings of the National Academy of Sciences of the United States of America, 1994, Aug-02, Volume: 91, Issue:16

Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of beta-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph.

Topics: Acetates; Acetonitriles; Buffers; Carbohydrate Sequence; Cellobiose; Cellulase; Cellulose; Cellulose 1,4-beta-Cellobiosidase; Electrons; Glycoside Hydrolases; Gold Colloid; Microscopy, Electron; Molecular Sequence Data; Scattering, Radiation; Staining and Labeling; Trichoderma

1994