cefoselis and sitafloxacin

Preliminary susceptibility testing interpretive criteria were determined for two investigational drugs, DU-6859a and FK-037, when tested against Haemophilus influenzae and Neisseria gonorrhoeae strains. National Committee for Clinical Laboratory Standards (NCCLS) methods with the Haemophilus Test Medium and GC agar base were utilized, and the study design conformed to the NCCLS M23-A guidelines. The proposed H. influenzae susceptibility testing criteria were for DU-6859a susceptible at < or = 1 microgram/ml (5-micrograms disk zone diameter correlate, > or = 19 mm) or < or = 2 micrograms/ml (disk zone diameter correlate, > or = 16 mm) and for FK-037 susceptible at < or = 2 micrograms/ml (30-micrograms disk zone diameter correlate, > or = 19 mm). No other susceptibility categories were proposed. The gonococcal susceptibility testing criteria were for DU-6859a susceptible at < or = 0.12 microgram/ml (disk zone diameter correlate, > or = 34 mm) and for FK-037 susceptible at < or = 0.5 microgram/ml (disk zone diameter correlate, > or = 30 mm) and resistant at > 1 microgram/ml (disk zone diameter correlate, < or = 26 mm). No resistant criterion was proposed for N. gonorrhoeae tests with DU-6859a. No interpretive discrepancies were observed between methods using these proposed testing criteria.

Topics: Anti-Infective Agents; Ceftizoxime; Fluoroquinolones; Haemophilus influenzae; Microbial Sensitivity Tests; Neisseria gonorrhoeae; Quinolones; Spiro Compounds

Several multilaboratory collaborative studies using broth microdilution and agar dilution (gonococcal tests only) methods of susceptibility testing were performed to establish quality-control (QC) giudelines. Replicate dilution tests with multiple lots of media were performed with National Committee for Clinical Laboratory Standards recommended QC strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, and Staphylococcus aureus ATCC 29213) by using the following antimicrobials: biapenem, DU-6859a, FK-037, levofloxacin, and grepafloxacin. In addition QC MIC ranges, using appropriate medium modification for Haemophilus influenzae ATCC 49247 and Neisseria gonorrhoeae ATCC 49226 were reported on four (DU-6859a, FK-037, levofloxacin, and grepafloxacin) and two (ceftizoxime and FK-037) antimicrobials, respectively.

Topics: Anti-Bacterial Agents; Anti-Infective Agents; Bacteria; Ceftizoxime; Fluoroquinolones; Levofloxacin; Microbial Sensitivity Tests; Ofloxacin; Piperazines; Quality Control; Quinolones; Reference Values; Spiro Compounds; Thienamycins