cefamandole and flomoxef

1. We evaluated in vitro inhibitory effects of six types of antibiotic, aztreonam (AZT), cefamandole (CMD), cefmetazole (CMZ), cefotiam (CTM), flomoxef (FMOX) and latamoxef (LMOX), on platelet aggregation, using healthy volunteers' blood. Four types--FMOX, LMOX, CTM and CMD--inhibited, in concentration of 2500 micrograms/ml, the secondary aggregation induced by 3.0 microM adenosine diphosphate (ADP), and also inhibited the aggregation induced by 0.5 micrograms/mi collagen. AZT in the same concentration, did not inhibit the aggregation induced by collagen, and it inhibited only ADP-induced aggregation. CMZ, in the same concentration, inhibited neither of the two aggregations. 2. The inhibitory effects of the antibiotics on collagen-induced aggregation were dependent on the concentration of respective antibiotics. When classified by the strength of inhibitory action, LMOX and FMOX were strong, followed by CTM and CMD. The action of AZT and CMZ was weak. In particular, LMOX showed a 32% inhibitory effect at concentration of 50 micrograms/ml, a level near the blood concentration obtained with clinical usual dose. 3. No relationship was observed between inhibitory effects of antibiotics on ADP- or collagen-induced aggregation and the presence or absence of carboxyl group and/or N-methyltetrazolethiol group in the chemical structure.

Topics: Adult; Anti-Bacterial Agents; Blood Platelets; Cefamandole; Cephalosporins; Collagen; Female; Humans; Male; Middle Aged; Moxalactam; Platelet Aggregation Inhibitors

Biological studies were done on numbers of methicillin-resistant strains of Staphylococcus aureus (MRSA) either clinically isolated at medical institutes nationwide and sent to us or isolated and identified by us from samples obtained in the year 1990 from patients with various infections. The results of the studies are summarized as follows. 1. The origins of the 1,047 strains used in our studies included arterial and venous blood samples (6.9%), samples from the respiratory tract (43.3%), surgical and dermatological samples (30.2%), otorhinolaryngological and ophthalmological samples (3.8%), urological samples (7.2%), fecal samples (5.1%) and others (3.6%), thus samples from the respiratory tract were the most frequent. 2. The strains were classified according to coagulase types into type II (76.4%), type III (2.1%), type IV (15.4%), type VII (5.3%) and other types (0.8%), thus type II strains were the most numerous. When classified according to their origins, type II strains were found in a significantly high frequency in respiratory tract samples, and type IV strains appeared to be present at relatively high frequencies in the surgical, dermatological, otorhinolaryngological and ophthalmological samples. Some coagulase types were found more frequently in samples from some institutes than in others, but coagulase type distributions were similar in different localities. 3. The strains were classified according to their enterotoxin types into type A (22.8%), type B (10.5%), type C (54.9%) and other types (11.7%), thus type C strains were the most frequent. Significant correlations were observed between enterotoxin type C and coagulase type II, and type A and type IV. 4. Defining strains with minimum inhibitory concentrations (MICs) of greater than or equal to 100 micrograms/ml as high MRSA and less than or equal to 50 micrograms/ml as moderate MRSA, 94.8% of coagulase type II strains were high MRSA and 78.9% of coagulase type IV strains were moderate MRSA, thus coagulase type II strains tended to be high in methicillin resistance. 5. MIC50 and MIC90 of vancomycin (VCM) against many of these strains of MRSA were 0.78 and 1.56 micrograms/ml, respectively, suggesting that VCM has also a potent antibacterial activity against recent isolates of MRSA in Japan.

Topics: Cefamandole; Cephalosporins; Cephalothin; Humans; Methicillin Resistance; Microbial Sensitivity Tests; Staphylococcus aureus; Vancomycin

Using newly invented method for collecting vitreous samples, we examined the permeability of antibiotics across the blood-ocular barrior in human eyes. One of the advantages of the present method is that it yields sufficient amounts of sample. This makes it possible to examine and compare the permeability of multiple drugs with the same sample. We studied two antibiotics, Flomoxef Sodium (FMOX) and Cefamandole Sodium (CMD) with proliferative vitreoretinopathy (8 cases), proliferative diabetica retinopathy (8 cases) and simple vitreous haemorrhage (9 cases: including 3 cases of Terson's syndrome). A considerable amount of FMOX was detected in the vitreous sample and the permeability of FMOX was 50% better than that of CMD. Permeability also differed recording of different ocular diseases. The amount of the drug was lowest in cases with simple vitreous hemorrhage, especially in Terson's syndrome (p less than 0.05). This is reasonable as the blood-ocular barrior must be less disturbed in this disease than the other two conditions.

Topics: Adult; Aged; Cefamandole; Cephalosporins; Diabetic Retinopathy; Eye Diseases; Female; Humans; Male; Middle Aged; Retinal Diseases; Vitreous Body

Incubation of isolated rat hepatocytes with high concentration of beta-lactam antibiotics, cephaloridine (CER), flomoxef (FMOX) or cephamandole (CMD), resulted in significant reduction of cellular glutathione (GSH) levels, though cell viability was not affected during the incubation period. I.v. injection of a large dose, 300 mg/kg, of these beta-lactam antibiotics to rats did not affect the hepatic GSH levels. The concentration of beta-lactam antibiotics in the body fluid of the rats were found to be much lower than the amounts which causes GSH depletion in vitro.

Topics: Animals; Anti-Bacterial Agents; Cefamandole; Cell Survival; Cephaloridine; Cephalosporins; Glutathione; Liver; Male; Rats; Rats, Inbred Strains