cdw17-antigen and ketodihydrosphingosine

cdw17-antigen has been researched along with ketodihydrosphingosine* in 2 studies

Other Studies

2 other study(ies) available for cdw17-antigen and ketodihydrosphingosine

Article	Year
Identification of a novel L-serine analog that suppresses osteoclastogenesis in vitro and bone turnover in vivo. The Journal of biological chemistry, 2009, Dec-04, Volume: 284, Issue:49 Osteoclasts are multinucleated giant cells with bone resorbing activity. We previously reported that the expression of the transcription factor NFAT2 (NFATc1) induced by receptor activator of NF-kappaB ligand (RANKL) is essential for the formation of multinucleated cells. We subsequently identified L-Ser in the differentiation medium as necessary for the expression of NFAT2. Here we searched for serine analogs that antagonize the function of L-Ser and suppress the formation of osteoclasts in bone marrow as well as RAW264 cells. An analog thus identified, H-Ser(tBu)-OMe x HCl, appeared to suppress the production of 3-ketodihydrosphingosine by serine palmitoyltransferase, and the expression and localization of RANK, a cognate receptor of RANKL, in membrane lipid rafts was down-regulated in the analog-treated cells. The addition of lactosylceramide, however, rescued the osteoclastic formation. When administered in vivo, the analog significantly increased bone density in mice and prevented high bone turnover induced by treatment with soluble RANKL. These results demonstrate a close connection between the metabolism of L-Ser and bone remodeling and also the potential of the analog as a novel therapeutic tool for bone destruction. Topics: Animals; Antigens, CD; Bone and Bones; Bone Density; Bone Marrow Cells; Esters; Female; Gene Expression Regulation; Giant Cells; In Vitro Techniques; Lactosylceramides; Membrane Microdomains; Mice; Mice, Inbred C57BL; NFATC Transcription Factors; Osteoclasts; RANK Ligand; Serine; Serine C-Palmitoyltransferase; Sphingosine	2009
Occurrence of ceramides and neutral glycolipids with unusual long-chain base composition in purified rat liver mitochondria. FEBS letters, 2001, Jan-19, Volume: 488, Issue:3 The free ceramide content of rat liver mitochondria was found to be 1.7 nmol/mg protein and outer membranes contained a three-fold higher concentration than inner membranes. The mitochondrial content in neutral glycolipids was 0.6 nmol/mg protein. The long-chain bases found in free ceramides were d18:1 sphingosine, d18:0 3-ketosphinganine and t21:1 phytosphingosine in increasing order. In contrast, 3-ketosphinganine was the only base of glucosylceramide and lactosylceramide of inner membranes, whereas d18:1 sphingosine was the major long-chain base of glucosylceramide of outer membranes. Topics: Animals; Antigens, CD; Ceramides; Fatty Acids; Gas Chromatography-Mass Spectrometry; Glucosylceramides; Intracellular Membranes; Lactosylceramides; Mitochondria, Liver; Neutral Glycosphingolipids; Rats; Sphingosine	2001

Article

Year

Identification of a novel L-serine analog that suppresses osteoclastogenesis in vitro and bone turnover in vivo.

The Journal of biological chemistry, 2009, Dec-04, Volume: 284, Issue:49

Osteoclasts are multinucleated giant cells with bone resorbing activity. We previously reported that the expression of the transcription factor NFAT2 (NFATc1) induced by receptor activator of NF-kappaB ligand (RANKL) is essential for the formation of multinucleated cells. We subsequently identified L-Ser in the differentiation medium as necessary for the expression of NFAT2. Here we searched for serine analogs that antagonize the function of L-Ser and suppress the formation of osteoclasts in bone marrow as well as RAW264 cells. An analog thus identified, H-Ser(tBu)-OMe x HCl, appeared to suppress the production of 3-ketodihydrosphingosine by serine palmitoyltransferase, and the expression and localization of RANK, a cognate receptor of RANKL, in membrane lipid rafts was down-regulated in the analog-treated cells. The addition of lactosylceramide, however, rescued the osteoclastic formation. When administered in vivo, the analog significantly increased bone density in mice and prevented high bone turnover induced by treatment with soluble RANKL. These results demonstrate a close connection between the metabolism of L-Ser and bone remodeling and also the potential of the analog as a novel therapeutic tool for bone destruction.

Topics: Animals; Antigens, CD; Bone and Bones; Bone Density; Bone Marrow Cells; Esters; Female; Gene Expression Regulation; Giant Cells; In Vitro Techniques; Lactosylceramides; Membrane Microdomains; Mice; Mice, Inbred C57BL; NFATC Transcription Factors; Osteoclasts; RANK Ligand; Serine; Serine C-Palmitoyltransferase; Sphingosine

2009

Occurrence of ceramides and neutral glycolipids with unusual long-chain base composition in purified rat liver mitochondria.

FEBS letters, 2001, Jan-19, Volume: 488, Issue:3

The free ceramide content of rat liver mitochondria was found to be 1.7 nmol/mg protein and outer membranes contained a three-fold higher concentration than inner membranes. The mitochondrial content in neutral glycolipids was 0.6 nmol/mg protein. The long-chain bases found in free ceramides were d18:1 sphingosine, d18:0 3-ketosphinganine and t21:1 phytosphingosine in increasing order. In contrast, 3-ketosphinganine was the only base of glucosylceramide and lactosylceramide of inner membranes, whereas d18:1 sphingosine was the major long-chain base of glucosylceramide of outer membranes.

Topics: Animals; Antigens, CD; Ceramides; Fatty Acids; Gas Chromatography-Mass Spectrometry; Glucosylceramides; Intracellular Membranes; Lactosylceramides; Mitochondria, Liver; Neutral Glycosphingolipids; Rats; Sphingosine

2001