cardiovascular-agents and cesium-chloride

Diaphragmatic lymphatic function is mainly sustained by pressure changes in the tissue and serosal cavities during cardiorespiratory cycles. The most peripheral diaphragmatic lymphatics are equipped with muscle cells (LMCs), which exhibit spontaneous contraction, whose molecular machinery is still undetermined. Hypothesizing that spontaneous contraction might involve hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in lymphatic LMCs, diaphragmatic specimens, including spontaneously contracting lymphatics, were excised from 33 anesthetized rats, moved to a perfusion chamber containing HEPES-Tyrode's solution, and treated with HCN channels inhibitors cesium chloride (CsCl), ivabradine, and ZD-7288. Compared with control, exposure to 10 mM CsCl reduced (-65%, n = 13, P < 0.01) the contraction frequency (F

Topics: Animals; Benzazepines; Cardiovascular Agents; Cesium; Chlorides; Diaphragm; Female; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; Immunohistochemistry; Ivabradine; Lymphatic Vessels; Male; Muscle, Smooth, Vascular; Myocytes, Smooth Muscle; Potassium Channels; Pyrimidines; Rats; Rats, Wistar; Real-Time Polymerase Chain Reaction; Vasoconstriction