carbocyanines and methyl-red

Asymmetric dye clusters with a single fluorophore (Cy3) and multiple quenchers (4'-methylthioazobenzene-4-carboxylate, methyl red, and 4'-dimethylamino-2-nitroazobenzene-4-carboxylate) were prepared. The dye and one-to-five quenchers were tethered through D-threoninol to opposite strands of a DNA duplex. NMR analysis revealed that the clusters with a single fluorophore and two quenchers formed a sandwich-like structure (antiparallel H-aggregates). The melting temperatures of all the heteroclusters were almost the same, although structural distortion should become larger, as the number of quenchers increased. An asymmetric heterocluster of a single fluorophore and two quenchers showed larger excitonic interaction (i.e., hypochromicity of Cy3), than did a single Cy3 and a single quencher. Due to the larger exciton coupling between the dyes, the 1:2 heterocluster suppressed the background emission more efficiently than the 1:1 cluster. However, more quenchers did not enhance quenching efficiency due to the saturation of exciton coupling with two quenchers. Finally, this asymmetric 1:2 heterocluster was introduced into the stem region of a molecular beacon (MB; also known as an in-stem MB) targeting the fusion site in the L6 BCR-ABL fusion gene. With this MB design, the signal/background ratio was as high as 68 due to efficient suppression of background emission resulting from the maximized excitonic interaction.

Topics: Amino Alcohols; Azo Compounds; Base Sequence; Butylene Glycols; Carbocyanines; Carboxylic Acids; DNA; Fluorescent Dyes; Genes, abl; Humans; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Conformation; Molecular Probes; Molecular Sequence Data; Nucleic Acid Denaturation; Organic Chemicals; Spectrometry, Fluorescence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature

An electrostatically self-assembled film of PMo12/H6 has successfully been prepared on quartz substrates by alternating adsorption of [PMo12O40(3-) (PMo12) and a bipolar hemicyanine derivative of (E)-1,1'-(hexane-1,6-diyl)bis(4-(4-(dimethylamino)styryl)pyridinium) bromide (H6Br2). The UV-visible spectra showed that the film was uniformly deposited and the charge transfer between two film-forming components might occur in the film. The photocatalytic performance of the film was studied for the degradation of aqueous dye Methyl Red (MR) under UV irradiation. The degradation of MR follows Langmuir-Hinshelwood first-order kinetics.

Topics: Adsorption; Azo Compounds; Carbocyanines; Cations; Linear Models; Nanostructures; Photochemical Processes; Spectrophotometry, Ultraviolet; Static Electricity; Tungsten Compounds