carbocyanines and chlorin

Glioblastoma multiforme (GBM) is the most common primary intracranial malignancy; survival can be improved by maximizing the extent-of-resection.. A near-infrared fluorophore (Indocyanine-Green, ICG) was combined with a photosensitizer (Chlorin-e6, Ce6) on the surface of superparamagnetic-iron-oxide-nanoparticles (SPIONs), all FDA-approved for clinical use, yielding a nanocluster (ICS) using a microemulsion. The physical-chemical properties of the ICS were systematically evaluated. Efficacy of photodynamic therapy (PDT) was evaluated in vitro with GL261 cells and in vivo in a subtotal resection trial using a syngeneic flank tumor model. NIR imaging properties of ICS were evaluated in both a flank and an intracranial GBM model.. A multimodal theragnostic agent comprised solely of FDA-approved components was developed to couple optical imaging and PDT. The findings demonstrated evidence for the potential theragnostic benefit of ICS in surgical oncology that is conducive to clinical integration.

Topics: Animals; Apoptosis; Carbocyanines; Cell Proliferation; Coloring Agents; Combined Modality Therapy; Female; Fluorescence; Glioblastoma; Humans; Mice; Mice, Inbred C57BL; Nanoparticles; Neurosurgical Procedures; Photochemotherapy; Porphyrins; Surgery, Computer-Assisted; Theranostic Nanomedicine; Tumor Cells, Cultured; Xenograft Model Antitumor Assays