carbocyanines and 2-(2-hydroxyphenyl)benzothiazole

HBT-Cy 1 has been previously reported as a highly selective fluorescent probe for lysosome visualization in live cells. To further investigate the role of the structural components of HBT-Cy in lysosome selectivity, cyanine based fluorescent probe series (2-5) have been synthesized in good yields by connecting benzothiazolium cyanine (Cy) with 2-hydroxyphenylbenzothiazole (HBT) via a meta phenylene ring. Probes 2-5 exhibited exceptional photophysical properties including bright red-emission (λ

Topics: Benzothiazoles; Biocompatible Materials; Carbocyanines; Cell Line; Cell Survival; Fluorescent Dyes; Humans; Hydrogen-Ion Concentration; Lysosomes; Microscopy, Confocal; Phenols; Temperature

A novel HBT-hemicyanine hybrid was prepared. This hybrid not only displays a large red-shifted (Δλ = 125 nm) emission compared to the well known ESIPT dye HBT, but also can be used as a new probe for rapid, colorimetric and ratiometric fluorescent detection of bisulfite with high selectivity and sensitivity in aqueous solution. The detection limit of this probe for HSO3(-) was calculated to be about 56 nM with a linear range of 0-25 μM. The potential application of this probe was exampled by detection of bisulfite in real food samples and living cells. Overall, this work not only provided a new ratiometric sensing platform, but also provided a new promising colorimetric and ratiometric fluorescent probe for bisulfite.

Topics: Benzothiazoles; Carbocyanines; Colorimetry; Coloring Agents; Dietary Sucrose; Fluorescent Dyes; Food Analysis; HeLa Cells; Humans; Limit of Detection; Phenols; Spectrometry, Fluorescence; Sulfites; Wine