cannabidiol and 4-hydroxy-2-nonenal

Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated.

Topics: Aldehydes; Anti-Inflammatory Agents; Antioxidants; Cannabidiol; Cell Culture Techniques; Cells, Cultured; Drug Evaluation, Preclinical; Gene Expression Regulation; Humans; I-kappa B Kinase; Keratinocytes; Molecular Structure; Multiprotein Complexes; NF-kappa B; Principal Component Analysis; Proteome; Signal Transduction; Tumor Necrosis Factor-alpha; Ultraviolet Rays