candicidin and hachimycin

It was shown that sulfur and phosphorus compounds (sodium thiosulfate, sodium tripolyphosphate, sodium hexametaphosphate, monosodium phosphate) catalyze cis-trans isomerization of aromatic heptaens. Preparative method of levorin isomerezation at the presence of sodium thiosulfate was elaborated. The isolated product was a fully trans-isomer.

Topics: Anti-Bacterial Agents; Antifungal Agents; Candicidin; Catalysis; cis-trans-Isomerases; Organic Chemicals; Phosphorus Compounds; Solutions; Spectrophotometry, Infrared; Stereoisomerism; Sulfur Compounds

Previous studies to find drugs with an existing product licence which were active at 4 degrees C within 24 hours and which would be suitable to prevent the transmission of Chagas' disease during blood transfusion were unsuccessful. As part of an alternative approach to identify drugs active at 37 degrees C or 25 degrees C within 2 hours, over 280 compounds were screened against bloodstream trypomastigotes of Trypanosoma cruzi Sonya strain in a microslide test in vitro. Although compounds from a wide range of chemical groups were tested only three polyene antibiotics showed outstanding trypanocidal activity. Amphotericin B, candicidin and trichomycin lysed all trypomastigotes at 8 X 10(-6) as determined by microscopical techniques. However, these compounds also showed toxicity to mammalian erythrocytes at the same concentration after a 24 hours incubation period. It seems unlikely that this approach will yield a candidate replacement for gentian violet.

Topics: Amphotericin B; Animals; Antifungal Agents; Blood; Candicidin; Drug Evaluation, Preclinical; Erythrocytes; Gentian Violet; Humans; Larva; Male; Mice; Mice, Inbred BALB C; Organic Chemicals; Temperature; Trypanocidal Agents; Trypanosoma cruzi

High performance liquid chromatographic (HPLC) procedures were utilized for the rapid and efficient separation and characterization of the aromatic heptaene macrolide group of antifungal antibiotics. The instrument utilized a 350 nm phosphor converted ultraviolet detector and a mu Bondapak C18 column packing. Optimum resolution of eleven commercial aromatic heptaene macrolide preparations was obtained with solvent systems consisting of mixtures of acetonitrile and 0.05 M aqueous sodium citrate buffer, pH 5.3. The presence of two distinct types of aromatic heptaene macrolides with numerous well-defined individual components has been established.

Topics: Antifungal Agents; Candicidin; Chromatography, High Pressure Liquid; Organic Chemicals; Polyenes

Topics: Amphotericin B; Anti-Bacterial Agents; Antifungal Agents; Candicidin; Dermatologic Agents; Fungi; Griseofulvin; Metabolism; Nystatin; Organic Chemicals; Pharmacology; Saccharomyces; Trichophyton

Topics: Actinobacteria; Actinomyces; Anti-Bacterial Agents; Antifungal Agents; Candicidin; Chromium; Fluorocarbons; Humans; Nystatin; Organic Chemicals; Polyenes; Streptomyces