camelliaside-a has been researched along with astragalin* in 2 studies
2 other study(ies) available for camelliaside-a and astragalin
| Article | Year |
|---|---|
Novel synthesis of leucoside by enzymatic hydrolysis of tea seed extract.
The application of tea seed extract (TSE) has been widely investigated owing to its biological activities. In this paper, two flavonol triglycosides found in TSE, camelliaside A (CamA) and camelliaside B (CamB), were subjected to hydrolysis in the presence of three commercial enzyme complexes of the Pectinex® series, 5XL, XXL and Ultra SP-L (Ultra).. XXL and 5XL induced stepwise deglycosylation of CamA and CamB to yield kaempferol diglycoside (nicotiflorin), kaempferol monoglycoside (astragalin) and kaempferol, while Ultra produced an additional new compound (1) that had not been observed in earlier studies. Upon hydrolysis of isolated CamA and CamB, compound (1) was obtained only from CamB. Both the molecular ion peak in liquid chromatography/mass spectrometry and the ¹H and ¹³C nuclear magnetic resonance spectra of (1) isolated by Ultra-induced hydrolysis of TSE indicated that (1) was kaempferol 3-O-β-xylopyranosyl (1 → 2)-β-glucopyranoside (leucoside), formed by selective hydrolysis of the rhamnosyl moiety of CamB.. Pure leucoside can be prepared by enzymatic partial hydrolysis of TSE. This is the first study to address the synthesis of pure leucoside from a natural source. Topics: Antioxidants; Aspergillus; Camellia sinensis; Chromatography, High Pressure Liquid; Drug Discovery; Flavonoids; Flavonols; Fungal Proteins; Glycosides; Glycosylation; Hydrolysis; Kaempferols; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Phenols; Plant Extracts; Quercetin; Seeds; Spectrometry, Mass, Electrospray Ionization | 2013 |
Isolation and anti-inflammatory effect of astragalin synthesized by enzymatic hydrolysis of tea seed extract.
The application of tea seed extract (TSE) has been widely investigated because of its biological activities. In this paper, two flavonol triglycosides in TSE-camelliaside A (CamA) and camelliaside B (CamB)-were subjected to hydrolysis in the presence of two commercial enzyme complexes (Pectinex™ series): Smash and Mash.. Smash hydrolyzed only the xylosyl moiety of CamB, and the main product was kaempferol diglycoside (nicotiflorin, NF). On the other hand, Mash induced the hydrolysis of both CamA and CamB, and kaempferol monoglycoside (astragalin, AS) was found to be a main product. Pure AS with > 96% purity was prepared by enzymatic hydrolysis of TSE using Mash, and the chemical structure of AS was confirmed by (1)H- and (13)C-nuclear magnetic resonance analyses. The prepared pure AS showed anti-inflammatory activities by significantly inhibiting cellular nitrite oxide (IC(50) = 363 µg mL(-1)), prostaglandin E(2) (IC(50) = 134 µg mL(-1)) and interleukin-6 production (IC(50) = 289 µg mL(-1)) by lipopolysaccharide -stimulated RAW 264.7 cells.. It was concluded that pure AS can be prepared by enzymatic partial hydrolysis of TSE and employed as an anti-inflammatory material. This is the first study to address the preparation of pure AS from natural sources. Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Camellia sinensis; Cell Line, Transformed; Dinoprostone; Fungal Proteins; Glycoside Hydrolases; Glycosides; Hydrolysis; Interleukin-6; Kaempferols; Lipopolysaccharides; Macrophages; Magnetic Resonance Spectroscopy; Mice; Nitric Oxide; Plant Extracts; Quercetin; Seeds | 2011 |