calyculin-a and adociasulfate-2

The unicellular green alga Micrasterias denticulata performs a two-directional postmitotic nuclear migration during development, a passive migration into the growing semicell, and a microtubule mediated backward migration towards the cell centre. The present study provides first evidence for force generation by motor proteins of the kinesin family in this process. The new kinesin specific inhibitor adociasulfate-2 causes abnormal nuclear displacement at 18 microM. AMP-PNP, a non hydrolyseable ATP analogue or the general ATPase inhibitors calyculin A and sodium orthovanadate also disturb nuclear migration. In addition kinesin-like proteins are detected by means of immunoblotting using antibodies against brain kinesin, plant derived antibodies to kinesin-like proteins and a calmodulin binding kinesin-like protein. Immunoelectron microscopy suggests a correlation of conventional kinesin-like proteins, but not of the calmodulin binding kinesin-like protein to the microtubule apparatus associated with the migrating nucleus.

Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Adenylyl Imidodiphosphate; Blotting, Western; Cell Movement; Cell Nucleus; Chlorophyta; Enzyme Inhibitors; Immunoblotting; Immunohistochemistry; Kinesins; Marine Toxins; Microscopy, Immunoelectron; Microtubules; Mitosis; Oxazoles; Sulfuric Acid Esters; Vanadates