calycosin-7-o-beta-d-glucopyranoside and gamma-sitosterol

To study the chemical constituents of aerial parts of Ammopiptanthus mongolicus.. Isolation and purification were carried out on silica gel, Sephadex LH-20 and HPLC column chromatography. The structures of the compounds were identified by physico-chemical properties and spectral analysis.. Nine compounds were isolated and identified as (+)-maackiain (1), brevifolin (2), 7-hydroxy-4'-methoxy isoflavanone (3), daidzein 4',7-diglucoside (4), genistein 4', 7-di-O-beta-D-glucoside (5), isolupalbigenin (6), ononin (7), beta-sitosterol (8), beta-daucosterol (9).. Compounds 2, 4 - 6 were obtained from the genus Ammopiptanthus for the first time.

Topics: Chromatography, Agarose; Chromatography, High Pressure Liquid; Fabaceae; Glucosides; Isoflavones; Plant Extracts; Plant Leaves; Pterocarpans; Silica Gel; Sitosterols; Taxoids