calcimycin and janus-green

calcimycin has been researched along with janus-green* in 2 studies

Other Studies

2 other study(ies) available for calcimycin and janus-green

Article	Year
Low levels of cadmium chloride damage the corneal endothelium. Archives of toxicology, 1997, Volume: 71, Issue:7 The effect of cadmium chloride on the integrity of the endothelium of isolated bullfrog (Rana catesbeiana) corneas was examined by spectrophotometric analysis of corneal uptake of the vital stain Janus green and by scanning electron microscopy (SEM). The uptake of Janus green by the endothelium was dose related between 1.0 and 100.0 microM CdCl2. The effect of cadmium was significantly attenuated by the calcium channel blocker SKF 96365 and was augmented by the calcium ionophore A23187, indicating that cadmium influx through calcium channels is an important determinant of its cellular effect. The effect of cadmium was not altered by changes in the external calcium concentration, indicating that the mechanism does not involve competitive inhibition by calcium. SEM demonstrated significant structural damage to the corneal endothelium exposed to cadmium chloride, including focal disruption and denuding of the apical endothelial membrane. Topics: Animals; Azo Compounds; Cadmium Chloride; Calcimycin; Calcium Channel Blockers; Calcium Chloride; Coloring Agents; Dose-Response Relationship, Drug; Endothelium, Corneal; Imidazoles; Microscopy, Electron, Scanning; Rana catesbeiana	1997
Low levels of inorganic mercury damage the corneal endothelium. Experimental eye research, 1993, Volume: 57, Issue:5 The effect of inorganic mercury on the integrity of the endothelium of isolated bullfrog (Rana catesbeiana) corneas was examined by spectrophotometric analysis of corneal uptake of the vital stain Janus green, and by both transmission (TEM) and scanning (SEM) electron microscopy. The uptake of Janus green by the endothelium is dose related between 1.0 and 30.0 microM HgCl2. The effect of mercury is not altered by changes in external calcium concentration, nor is it influenced by the calcium ionophore A23187, indicating that inorganic mercury damages the corneal endothelium through a mechanism which does not involve competition with external calcium or interaction with calcium channels. TEM and SEM demonstrate significant ultrastructural damage to the endothelium exposed to inorganic mercury, including cellular swelling, increased vacuolization, focal denuding of Descemet's membrane, and diminished integrity at the intercellular junctions. Topics: Animals; Azo Compounds; Calcimycin; Calcium; Cell Membrane; Coloring Agents; Dose-Response Relationship, Drug; Endothelium, Corneal; In Vitro Techniques; Mercury; Microscopy, Electron; Rana catesbeiana; Spectrophotometry	1993

Article

Year

Low levels of cadmium chloride damage the corneal endothelium.

Archives of toxicology, 1997, Volume: 71, Issue:7

The effect of cadmium chloride on the integrity of the endothelium of isolated bullfrog (Rana catesbeiana) corneas was examined by spectrophotometric analysis of corneal uptake of the vital stain Janus green and by scanning electron microscopy (SEM). The uptake of Janus green by the endothelium was dose related between 1.0 and 100.0 microM CdCl2. The effect of cadmium was significantly attenuated by the calcium channel blocker SKF 96365 and was augmented by the calcium ionophore A23187, indicating that cadmium influx through calcium channels is an important determinant of its cellular effect. The effect of cadmium was not altered by changes in the external calcium concentration, indicating that the mechanism does not involve competitive inhibition by calcium. SEM demonstrated significant structural damage to the corneal endothelium exposed to cadmium chloride, including focal disruption and denuding of the apical endothelial membrane.

Topics: Animals; Azo Compounds; Cadmium Chloride; Calcimycin; Calcium Channel Blockers; Calcium Chloride; Coloring Agents; Dose-Response Relationship, Drug; Endothelium, Corneal; Imidazoles; Microscopy, Electron, Scanning; Rana catesbeiana

1997

Low levels of inorganic mercury damage the corneal endothelium.

Experimental eye research, 1993, Volume: 57, Issue:5

The effect of inorganic mercury on the integrity of the endothelium of isolated bullfrog (Rana catesbeiana) corneas was examined by spectrophotometric analysis of corneal uptake of the vital stain Janus green, and by both transmission (TEM) and scanning (SEM) electron microscopy. The uptake of Janus green by the endothelium is dose related between 1.0 and 30.0 microM HgCl2. The effect of mercury is not altered by changes in external calcium concentration, nor is it influenced by the calcium ionophore A23187, indicating that inorganic mercury damages the corneal endothelium through a mechanism which does not involve competition with external calcium or interaction with calcium channels. TEM and SEM demonstrate significant ultrastructural damage to the endothelium exposed to inorganic mercury, including cellular swelling, increased vacuolization, focal denuding of Descemet's membrane, and diminished integrity at the intercellular junctions.

Topics: Animals; Azo Compounds; Calcimycin; Calcium; Cell Membrane; Coloring Agents; Dose-Response Relationship, Drug; Endothelium, Corneal; In Vitro Techniques; Mercury; Microscopy, Electron; Rana catesbeiana; Spectrophotometry

1993