calcimycin has been researched along with ethyl-maltol* in 1 studies
1 other study(ies) available for calcimycin and ethyl-maltol
| Article | Year |
|---|---|
Intracellular free zinc and zinc buffering in human red blood cells.
Zn2+ has been allowed to equilibrate across the red cell membrane using two agents that increase membrane permeability to this ion: the ionophore A23187 and the specific carrier ethylmaltol. Extracellular free Zn2+ was controlled with EGTA (1,2-di(2-aminoethoxy)ethane-NNN'N'tetra-acetic acid] buffers, except in the case of ethylmaltol, which itself acts as a buffer. Measurement of cellular zinc content at different levels of free Zn2+ facilitated the study of intracellular Zn2+ binding. It was also possible to estimate intracellular free Zn2+ concentration in untreated cells using a "null-point" technique. Intracellular zinc was found to consist of an inexchangeable component of about 129 mumol/10(13) cells and an exchangeable component of 6.7 +/- 1.5 mumol/10(13) cells, with a free concentration of about 2.4 x 10(-11) M. The main component of Zn2+ buffering is hemoglobin, with a dissociation constant of about 2 x 10(-8) M. Topics: Binding Sites; Buffers; Calcimycin; Cell Membrane Permeability; Egtazic Acid; Erythrocyte Membrane; Erythrocytes; Humans; Magnesium; Pyrones; Zinc | 1991 |