calcimycin and anthranilic-acid

calcimycin has been researched along with anthranilic-acid* in 1 studies

Other Studies

1 other study(ies) available for calcimycin and anthranilic-acid

ArticleYear
Mutasynthesis of pyrrole spiroketal compound using calcimycin 3-hydroxy anthranilic acid biosynthetic mutant.
    Applied microbiology and biotechnology, 2013, Volume: 97, Issue:18

    The five-membered aromatic nitrogen heterocyclic pyrrole ring is a building block for a wide variety of natural products. Aiming at generating new pyrrole-containing derivatives as well as to identify new candidates that may be of value in designing new anticancer, antiviral, and/or antimicrobial agents, we employed a strategy on pyrrole-containing compound mutasynthesis using the pyrrole-containing calcimycin biosynthetic gene cluster. We blocked the biosynthesis of the calcimycin precursor, 3-hydroxy anthranilic acid, by deletion of calB1-3 and found that two intermediates containing the pyrrole and the spiroketal moiety were accumulated in the culture. We then fed the mutant using the structurally similar compound of 3-hydroxy anthranilic acid. At least four additional new pyrrole spiroketal derivatives were obtained. The structures of the intermediates and the new pyrrole spiroketal derivatives were identified using LC-MS and NMR. One of them shows enhanced antibacterial activity. Our work shows a new way of pyrrole derivative biosynthetic mutasynthesis.

    Topics: Anti-Bacterial Agents; Bacterial Proteins; Biosynthetic Pathways; Calcimycin; Furans; Mass Spectrometry; Molecular Structure; Mutation; ortho-Aminobenzoates; Pyrroles; Spiro Compounds; Streptomyces

2013