calcimycin and anisodamine

calcimycin has been researched along with anisodamine* in 2 studies

Other Studies

2 other study(ies) available for calcimycin and anisodamine

Article	Year
[Inhibition of phorbol ester and A23187-stimulated prostaglandins synthesis in macrophages by anisodamine]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 1990, Volume: 12, Issue:4 The effect of anisodamine on arachidonic acid metabolism in macrophages was studied. The results indicated that the synthesis of PGI2, TXA2 and PGF2 alpha stimulated by phorbol-12-myristate-13-acetate (PMA) and calcium ionophore A23187 was dose and time-dependently inhibited by anisodamine, and preincubation of the cells with anisodamine significantly decreased subsequent PMA and A23187-stimulated synthesis of prostaglandins. An isodamine failed to inhibit exogenous arachidonic acid-stimulated synthesis of prostaglandins. It is suggested that the site of action of anisodamine could be the phospholipase A2 reaction. Topics: 6-Ketoprostaglandin F1 alpha; Animals; Anti-Inflammatory Agents, Non-Steroidal; Calcimycin; Macrophages; Mice; Peritoneal Cavity; Solanaceous Alkaloids; Tetradecanoylphorbol Acetate; Thromboxane B2	1990
[Inhibition of release of prostaglandins and leukotrienes from calcimycin-induced mouse peritoneal macrophages and bovine aorta endothelial cells by anisodamine]. Zhongguo yao li xue bao = Acta pharmacologica Sinica, 1989, Volume: 10, Issue:3 [3H]Arachidonic acid (AA)-prelabeled mouse peritoneal macrophages were stimulated by calcium ionophore A-23187 to release [3H]AA metabolites. The major labeled products which were co-chromatographed with the authentic PG and LT standards by TLC and determined by liquid scintillation were 6-keto-PGF1 alpha, PGE2, LTC4, and LTB4. Anisodamine (Ani) significantly inhibited the A-23187-induced release of PG and LT from mouse macrophages in a dose-dependent manner. In the presence of Ani at 0.5 mmol/L, the A-23187-induced release of 6-keto-PGF1 alpha, PGE2, LTC4 and LTB4 was reduced by 57%, 20%, 53% and 49%, respectively. A-23187-induced release of 6-keto-PGF1 alpha measured by RIA from bovine aorta endothelial cells was also significantly inhibited by Ani in a dose-dependent manner. These results indicate that the calcium-antagonistic effects of Ani may not only play a significant role in its inhibiting release of PG and LT, but also contribute to its salutary effects in the treatment of septic shock. Topics: 6-Ketoprostaglandin F1 alpha; Animals; Aorta; Calcimycin; Cattle; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular; Female; Leukotriene B4; Macrophages; Male; Mice; Peritoneal Cavity; Prostaglandins; Prostaglandins E; Solanaceous Alkaloids; SRS-A	1989

Article

Year

[Inhibition of phorbol ester and A23187-stimulated prostaglandins synthesis in macrophages by anisodamine].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 1990, Volume: 12, Issue:4

The effect of anisodamine on arachidonic acid metabolism in macrophages was studied. The results indicated that the synthesis of PGI2, TXA2 and PGF2 alpha stimulated by phorbol-12-myristate-13-acetate (PMA) and calcium ionophore A23187 was dose and time-dependently inhibited by anisodamine, and preincubation of the cells with anisodamine significantly decreased subsequent PMA and A23187-stimulated synthesis of prostaglandins. An isodamine failed to inhibit exogenous arachidonic acid-stimulated synthesis of prostaglandins. It is suggested that the site of action of anisodamine could be the phospholipase A2 reaction.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Anti-Inflammatory Agents, Non-Steroidal; Calcimycin; Macrophages; Mice; Peritoneal Cavity; Solanaceous Alkaloids; Tetradecanoylphorbol Acetate; Thromboxane B2

1990

[Inhibition of release of prostaglandins and leukotrienes from calcimycin-induced mouse peritoneal macrophages and bovine aorta endothelial cells by anisodamine].

Zhongguo yao li xue bao = Acta pharmacologica Sinica, 1989, Volume: 10, Issue:3

[3H]Arachidonic acid (AA)-prelabeled mouse peritoneal macrophages were stimulated by calcium ionophore A-23187 to release [3H]AA metabolites. The major labeled products which were co-chromatographed with the authentic PG and LT standards by TLC and determined by liquid scintillation were 6-keto-PGF1 alpha, PGE2, LTC4, and LTB4. Anisodamine (Ani) significantly inhibited the A-23187-induced release of PG and LT from mouse macrophages in a dose-dependent manner. In the presence of Ani at 0.5 mmol/L, the A-23187-induced release of 6-keto-PGF1 alpha, PGE2, LTC4 and LTB4 was reduced by 57%, 20%, 53% and 49%, respectively. A-23187-induced release of 6-keto-PGF1 alpha measured by RIA from bovine aorta endothelial cells was also significantly inhibited by Ani in a dose-dependent manner. These results indicate that the calcium-antagonistic effects of Ani may not only play a significant role in its inhibiting release of PG and LT, but also contribute to its salutary effects in the treatment of septic shock.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Aorta; Calcimycin; Cattle; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular; Female; Leukotriene B4; Macrophages; Male; Mice; Peritoneal Cavity; Prostaglandins; Prostaglandins E; Solanaceous Alkaloids; SRS-A

1989