calcimycin and acetoacetic-acid

calcimycin has been researched along with acetoacetic-acid* in 2 studies

Other Studies

2 other study(ies) available for calcimycin and acetoacetic-acid

Article	Year
Augmented production of platelet-activating factor in human polymorphonuclear leukocytes by ketone bodies. FEBS letters, 1989, Dec-04, Volume: 258, Issue:2 The production of platelet-activating factor (PAF) in A23187-stimulated human polymorphonuclear leukocytes was markedly increased in the presence of 5 mM acetoacetate and beta-hydroxybutyrate. Such an augmentation was observed even at 500 microM but not at 50 microM. The augmented production of PAF by acetoacetate was also observed in the presence of autologous serum and was most prominent in the case of opsonized zymosan-stimulation rather than A23187-stimulation. These observations suggest that increased levels of acetoacetate and beta-hydroxybutyrate in blood may lead to the augmented production of PAF, which would amplify the various PAF-mediated biological reactions. Topics: 3-Hydroxybutyric Acid; Acetoacetates; Calcimycin; Dimethyl Sulfoxide; Humans; Hydroxybutyrates; In Vitro Techniques; Kinetics; Neutrophils; Platelet Activating Factor; Tetradecanoylphorbol Acetate; Zymosan	1989
Is there Ca2+(Sr2+)-3-hydroxybutyrate symport in rat-liver mitochondria? A reappraisal. European journal of biochemistry, 1986, Jun-02, Volume: 157, Issue:2 The observation in this laboratory that respiration and Sr2+ import were stimulated by the addition of 3-hydroxybutyrate to suspensions of N-ethylmaleimide-treated mitochondria respiring in state 6, after the addition of Sr2+, in a sucrose medium containing choline as substrate, led to the proposal by Moyle and Mitchell [(1977) FEBS Lett. 84, 135-140] that there is a Ca2+(Sr2+)-3-hydroxybutyrate symporter in rat liver mitochondria. However, experiments described in the present paper support a different interpretation. Under the conditions of the experiments by Moyle and Mitchell, the rate of respiration and the poise of Sr2+ accumulation are mainly limited, not by delta mu H+, but by lack of respiratory substrate. Even though N-ethylmaleimide is a potent inhibitor of 3-hydroxybutyrate dehydrogenase, we have found that, somewhat surprisingly, under the special conditions of these experiments, sufficient 3-hydroxybutyrate dehydrogenase activity remains available to account for the 3-hydroxybutyrate-dependent respiratory stimulation and Sr2+ import. Topics: 3-Hydroxybutyric Acid; Acetoacetates; Animals; Biological Transport, Active; Butyrates; Butyric Acid; Calcimycin; Calcium; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; Ethylmaleimide; Hydrogen-Ion Concentration; Hydroquinones; Hydroxybutyrate Dehydrogenase; Hydroxybutyrates; Mitochondria, Liver; Oxygen Consumption; Rats; Rotenone; Strontium; Time Factors	1986

Article

Year

Augmented production of platelet-activating factor in human polymorphonuclear leukocytes by ketone bodies.

FEBS letters, 1989, Dec-04, Volume: 258, Issue:2

The production of platelet-activating factor (PAF) in A23187-stimulated human polymorphonuclear leukocytes was markedly increased in the presence of 5 mM acetoacetate and beta-hydroxybutyrate. Such an augmentation was observed even at 500 microM but not at 50 microM. The augmented production of PAF by acetoacetate was also observed in the presence of autologous serum and was most prominent in the case of opsonized zymosan-stimulation rather than A23187-stimulation. These observations suggest that increased levels of acetoacetate and beta-hydroxybutyrate in blood may lead to the augmented production of PAF, which would amplify the various PAF-mediated biological reactions.

Topics: 3-Hydroxybutyric Acid; Acetoacetates; Calcimycin; Dimethyl Sulfoxide; Humans; Hydroxybutyrates; In Vitro Techniques; Kinetics; Neutrophils; Platelet Activating Factor; Tetradecanoylphorbol Acetate; Zymosan

1989

Is there Ca2+(Sr2+)-3-hydroxybutyrate symport in rat-liver mitochondria? A reappraisal.

European journal of biochemistry, 1986, Jun-02, Volume: 157, Issue:2

The observation in this laboratory that respiration and Sr2+ import were stimulated by the addition of 3-hydroxybutyrate to suspensions of N-ethylmaleimide-treated mitochondria respiring in state 6, after the addition of Sr2+, in a sucrose medium containing choline as substrate, led to the proposal by Moyle and Mitchell [(1977) FEBS Lett. 84, 135-140] that there is a Ca2+(Sr2+)-3-hydroxybutyrate symporter in rat liver mitochondria. However, experiments described in the present paper support a different interpretation. Under the conditions of the experiments by Moyle and Mitchell, the rate of respiration and the poise of Sr2+ accumulation are mainly limited, not by delta mu H+, but by lack of respiratory substrate. Even though N-ethylmaleimide is a potent inhibitor of 3-hydroxybutyrate dehydrogenase, we have found that, somewhat surprisingly, under the special conditions of these experiments, sufficient 3-hydroxybutyrate dehydrogenase activity remains available to account for the 3-hydroxybutyrate-dependent respiratory stimulation and Sr2+ import.

Topics: 3-Hydroxybutyric Acid; Acetoacetates; Animals; Biological Transport, Active; Butyrates; Butyric Acid; Calcimycin; Calcium; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; Ethylmaleimide; Hydrogen-Ion Concentration; Hydroquinones; Hydroxybutyrate Dehydrogenase; Hydroxybutyrates; Mitochondria, Liver; Oxygen Consumption; Rats; Rotenone; Strontium; Time Factors

1986