calcein-am and resazurin

calcein-am has been researched along with resazurin* in 3 studies

Other Studies

3 other study(ies) available for calcein-am and resazurin

ArticleYear
Plasma mediated collagen-I-coating of metal implant materials to improve biocompatibility.
    Journal of biomedical materials research. Part A, 2010, Volume: 94, Issue:1

    This study describes the collagen-I coating of titanium and steel implants via cold low-pressure gas plasma treatment. To analyze the coatings in terms of biocompatibility osteoblast-like osteosarcoma cells and human leukocytes were cultivated on the metal surfaces. Two different implant materials were assessed (Ti6Al4V, X2CrNiMo18) and four different surface properties were evaluated: (a) plasma pretreated and collagen-I coated implant materials; (b) collagen-I dip-coated without plasma pretreatment; (c) plasma treated but not collagen-I coated; (d) standard implant materials served as control. The different coating characteristics were analyzed by scanning electron microscopy (SEM). For adhesion and viability tests calcein-AM staining of the cells and Alamar blue assays were performed. The quantitative analysis was conducted by computer assisted microfluorophotography and spectrometer measurements. SEM analysis revealed that stable collagen-I coatings could not be achieved on the dip-coated steel and titanium alloys. Only due to pretreatment with low-pressure gas plasma a robust deposition of collagen I on the surface could be achieved. The cell viability and cell attachment rate on the plasma pretreated, collagen coated surfaces was significantly (p < 0.017) increased compared to the non coated surfaces. Gas plasma treatment is a feasible method for the deposition of proteins on metal implant materials resulting in an improved biocompatibility in vitro. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

    Topics: Alloys; Animals; Cell Adhesion; Cell Proliferation; Cells, Cultured; Coated Materials, Biocompatible; Collagen Type I; Fluoresceins; Fluorescent Dyes; Humans; Indicators and Reagents; Leukocytes; Materials Testing; Oxazines; Prostheses and Implants; Stainless Steel; Surface Properties; Titanium; Xanthenes

2010
Limitations and relative utility of screening assays to assess engineered nanoparticle toxicity in a human cell line.
    Toxicology and applied pharmacology, 2009, Jan-15, Volume: 234, Issue:2

    Single-walled carbon nanotubes (SWCNT), fullerenes (C(60)), carbon black (CB), nC(60), and quantum dots (QD) have been studied in vitro to determine their toxicity in a number of cell types. Here, we report that classical dye-based assays such as MTT and neutral red (NR) that determine cell viability produce invalid results with some NM (nanomaterials) due to NM/dye interactions and/or NM adsorption of the dye/dye products. In this study, human epidermal keratinocytes (HEK) were exposed in vitro to CB, SWCNT, C(60), nC(60), and QD to assess viability with calcein AM (CAM), Live/Dead (LD), NR, MTT, Celltiter 96 AQueous One (96 AQ), alamar Blue (aB), Celltiter-Blue (CTB), CytoTox Onetrade mark (CTO), and flow cytometry. In addition, trypan blue (TB) was quantitated by light microscopy. Assay linearity (R(2) value) was determined with HEK plated at concentrations from 0 to 25,000 cells per well in 96-well plates. HEK were treated with serial dilutions of each NM for 24 h and assessed with each of the viability assays. TB, CAM and LD assays, which depend on direct staining of living and/or dead cells, were difficult to interpret due to physical interference of the NM with cells. Results of the dye-based assays varied a great deal, depending on the interactions of the dye/dye product with the carbon nanomaterials (CNM). Results show the optimal high throughput assay for use with carbon and noncarbon NM was 96 AQ. This study shows that, unlike small molecules, CNM interact with assay markers to cause variable results with classical toxicology assays and may not be suitable for assessing nanoparticle cytotoxicity. Therefore, more than one assay may be required when determining nanoparticle toxicity for risk assessment.

    Topics: Cell Line; Cell Survival; Drug Evaluation, Preclinical; Flow Cytometry; Fluoresceins; Fluorescent Dyes; Humans; Keratinocytes; Light; Microscopy, Electron, Transmission; Nanoparticles; Oxazines; Quantum Dots; Scattering, Radiation; Spectrophotometry, Ultraviolet; Tetrazolium Salts; Thiazoles; Trypan Blue; Xanthenes

2009
Amyloid beta-mediated oxidative and metabolic stress in rat cortical neurons: no direct evidence for a role for H2O2 generation.
    Journal of neurochemistry, 1996, Volume: 67, Issue:4

    H2O2 and free radical-mediated oxidative stresses have been implicated in mediating amyloid beta (1-40) [A beta (1-40)] neurotoxicity to cultured neurons. In this study, we confirm that addition of the H2O2-scavenging enzyme catalase protects neurons in culture against A beta-mediated toxicity; however, it does so by a mechanism that does not involve its ability to scavenge H2O2. A beta-mediated elevation in intracellular H2O2 production is suppressed by addition of a potent H2O2 scavenger without any significant neuroprotection. Three intracellular biochemical markers of H2O2-mediated oxidative stress were unchanged by A beta treatment: (a) glyceraldehyde-3-phosphate dehydrogenase activity, (b) hexose monophosphate shunt activity, and (c) glucose oxidation via the tricarboxylic acid cycle. lonspray mass spectra of A beta in the incubation medium indicated that A beta itself is an unlikely source of reactive oxygen species. In this study we demonstrate that intracellular ATP concentration is compromised during the first 24-h exposure of neurons to A beta. Our results challenge a pivotal role for H2O2 generation in mediating A beta toxicity, and we suggest that impairment of energy homeostasis may be a more significant early factor in the neurodegenerative process.

    Topics: Adenosine Triphosphate; Amyloid beta-Peptides; Analysis of Variance; Animals; Benzothiazoles; Catalase; Cell Survival; Cells, Cultured; Cerebral Cortex; Coloring Agents; Fetus; Fluoresceins; Free Radical Scavengers; Glucose; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycolysis; Glyoxylates; Hydrogen Peroxide; L-Lactate Dehydrogenase; Neurons; Neurotoxins; Oxazines; Oxidative Stress; Peptide Fragments; Rats; Reactive Oxygen Species; Thiazoles; Xanthenes

1996