calcein-am and ferulic-acid
calcein-am has been researched along with ferulic-acid* in 1 studies
Other Studies
1 other study(ies) available for calcein-am and ferulic-acid
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Ferulic acid reverses ABCB1-mediated paclitaxel resistance in MDR cell lines.
Multidrug resistance (MDR) remains a major obstacle in cancer chemotherapy. The use of the dietary phytochemicals as chemosensitizing agents to enhance the efficacy of conventional cytostatic drugs has recently gained the attention as a plausible approach for overcoming the drug resistance. The aim of this study was to investigate whether a naturally occurring diet-based phenolic acid, ferulic acid, could sensitize paclitaxel efficacy in ABCB1 overexpressing (P-glycoprotein) colchicine selected KB Ch(R)8-5 cell line. In vitro drug efflux assays demonstrated that ferulic acid inhibits P-glycoprotein transport function in drug resistant KB Ch(R)8-5 cell lines. However, ferulic acid significantly downregulates ABCB1 expression in a concentration dependent manner. Cytotoxicity assay reveals that ferulic acid decreased paclitaxel resistance in KBCh(R)8-5 and HEK293/ABCB1 cells, which indicates its chemosensitizing potential. Clonogenic cell survival assay and apoptotic morphological staining further confirm the chemosensitizing potential of ferulic acid in drug resistant KB Ch(R)8-5 cell lines. Ferulic acid treatment enhances paclitaxel mediated cell cycle arrest and upregulates paclitaxel-induced apoptotic signaling in KB resistant cells. Hence, it has been concluded that downregulation of ABCB1 and subsequent induction of paclitaxel-mediated cell cycle arrest and apoptotic signaling may be the cause for the chemosensitizing potential of ferulic acid in P-gp overexpressing cell lines. Topics: Apoptosis; ATP Binding Cassette Transporter, Subfamily B; Cell Movement; Cell Survival; Coumaric Acids; Drug Resistance, Multiple; Fluoresceins; G2 Phase Cell Cycle Checkpoints; Gene Expression Regulation, Neoplastic; Humans; KB Cells; M Phase Cell Cycle Checkpoints; Molecular Docking Simulation; Paclitaxel; Protein Conformation | 2016 |