butaprost and 8-bromoadenosine-3--5--cyclic-monophosphorothioate

butaprost has been researched along with 8-bromoadenosine-3--5--cyclic-monophosphorothioate* in 1 studies

Other Studies

1 other study(ies) available for butaprost and 8-bromoadenosine-3--5--cyclic-monophosphorothioate

ArticleYear
Role of Epac1 in mediating anti-proliferative effects of prostanoid EP(2) receptors and cAMP in human lung fibroblasts.
    Naunyn-Schmiedeberg's archives of pharmacology, 2008, Volume: 378, Issue:6

    In lung fibroblasts, proliferation is inhibited by activation of EP(2) prostanoid receptors which are known to couple to adenylyl cyclase. Beside the classic target of cAMP, protein kinase A (PKA), alternative cAMP effectors have been identified, among them Epac (exchange protein activated by cAMP). The present study aimed to illuminate transduction pathways mediating the anti-proliferative effects of EP(2) receptors in lung fibroblasts. Proliferative activity of human lung fibroblasts was determined by measuring [(3)H]-thymidine incorporation. The selective EP(2) receptor agonist butaprost inhibited [(3)H]-thymidine incorporation by 75%, an effect mimicked by forskolin, the phosphodiesterase inhibitor IBMX, the stable cAMP analogues dibutyryl-cAMP and bromo-cAMP, as well as by the Epac selective cAMP analogues 8-pCPT-2'-O-Me-cAMP and Sp-8-pCPT-2'-O-Me-cAMPS, whereas the PKA selective agonist 6-Bnz-cAMP was inactive. The PKA inhibitor Rp-8-Br-cAMPS inhibited butaprost-induced phosphorylation of CREB (cAMP response element-binding protein), but did not affect butaprost-induced inhibition of [(3)H]-thymidine incorporation. Partial knockdown of Epac1 by specific siRNA transfection resulted in a marked attenuation of the inhibitory potency of butaprost, whereas transfection of Epac2 siRNA or non-silencing siRNA did not affect the effectiveness of butaprost to inhibit [(3)H]-thymidine incorporation. In conclusion, Epac1 rather than the classic cAMP effector PKA is a crucial element in the signal transduction pathway mediating anti-proliferative effects of EP(2) receptor activation.

    Topics: 8-Bromo Cyclic Adenosine Monophosphate; Alprostadil; Cell Proliferation; Cells, Cultured; Collagen; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Enzyme Activation; Fibroblasts; Gene Knockdown Techniques; Guanine Nucleotide Exchange Factors; Humans; Lung; Phosphorylation; Receptors, Prostaglandin E; RNA, Small Interfering; Signal Transduction; Thionucleotides

2008