brucine and 2-3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline

Assessment of neural activity in the specific brain area is critical for understanding the circuit mechanisms underlying altered brain function and behaviors. A number of immediate early genes (IEGs) that are rapidly transcribed in neuronal cells in response to synaptic activity have been used as markers for neuronal activity. However, protein detection of IEGs requires translation, and the amount of newly synthesized gene product is usually insufficient to detect using western blotting, limiting their utility in western blot analysis of brain tissues for comparison of basal activity between control and genetically modified animals. Here, we show that the phosphorylation status of eukaryotic elongation factor-2 (eEF2) rapidly changes in response to synaptic and neural activities. Intraperitoneal injections of the GABA A receptor (GABA

Topics: Animals; Brain; CA3 Region, Hippocampal; Eukaryotic Initiation Factor-2; Genes, Reporter; Mice; Muscimol; Nerve Tissue Proteins; Phosphorylation; Picrotoxin; Prosencephalon; Protein Processing, Post-Translational; Pyramidal Cells; Quinoxalines; Restraint, Physical; Stress, Physiological; Strychnine