bromochloroacetic-acid and allyl-alcohol

Chemical modification of chicken feathers (CF) and their subsequent role in arsenic removal from water is presented in this paper. The ground CF were chemically treated with four selective dopants such as poly (ethylene glycol) (PEG) diglycidyl ether, poly (N-isopropylacrylamide) (PNIPAM), allyl alcohol (AA) and TrisilanolCyclohexyl POSS. After modification, the solubilized keratin was regenerated by precipitation at acidic pH. The structural changes and properties of modified biopolymer were compared with untreated CF and confirmed by different characterization techniques such as SEM, FTIR, XRD, and DSC. The TGA data was used to discuss thermal decomposition and kinetic behavior of modified biopolymer exhaustively. The modified biopolymers were further investigated as biosorbents for their application in As(III) removal from water. The AA and POSS supported biosorbents executed high removal capacity for As(III) up to 11.5 × 10(-2)and 11.0 × 10(-2)mg/g from 100ml arsenic polluted water solution respectively. Thermodynamic parameters such as ΔG(0), ΔH(0), ΔS(0) were also evaluated with the finding that overall sorption process was endothermic and spontaneous in nature. Based on linear and non-linear regression analysis, Freundlich Isotherm model showed good fit for obtained sorption data apart from high linear regression values supporting Langmuir isotherm model in sorption of As(III).

Topics: Acrylic Resins; Adsorption; Animals; Arsenic; Chickens; Epoxy Resins; Feathers; Hot Temperature; Hydrogen-Ion Concentration; Keratins; Organosilicon Compounds; Propanols; Thermogravimetry; Water Pollutants, Chemical; Water Purification

Several studies have demonstrated that bone marrow contains a subpopulation of stem cells capable of participating in the hepatic regenerative process, even if some reports indicate quite a low level of liver repopulation by human stem cells in the normal and transiently injured liver.. In order to overcome the low engraftment levels seen in previous models, we tried the direct intraperitoneal administration of human cord blood stem cells, using a model of hepatic damage induced by allyl alcohol in NOD/SCID mice.. We designed a protocol based on stem cell infusion following liver damage in the absence of irradiation. Flow cytometry, histology, immunohistochemistry and RT-PCR for human hepatic markers were performed to monitor human cell engraftment.. Human stem cells were able to transdifferentiate into hepatocytes, to improve liver regeneration after damage and to reduce the mortality rate both in both protocols, even if with qualitative and quantitative differences in the transdifferentiation process.. We demonstrated for the first time that the intraperitoneal administration of stem cells can guarantee a rapid liver engraftment. Moreover, the new protocol based on stem cell infusion following liver damage in the absence of irradiation may represent a step forward for the clinical application of stem cell transplantation.

Topics: Animals; Chemical and Drug Induced Liver Injury; Cord Blood Stem Cell Transplantation; Disease Models, Animal; Flow Cytometry; Glyceraldehyde-3-Phosphate Dehydrogenases; Humans; Immunohistochemistry; Keratin-7; Keratins; Liver; Mice; Mice, Inbred NOD; Mice, SCID; Propanols; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Survival Rate; Transplantation, Heterologous; Treatment Outcome