bromochloroacetic-acid and 5-bromo-4-chloro-3-indolyl-beta-galactoside

bromochloroacetic-acid has been researched along with 5-bromo-4-chloro-3-indolyl-beta-galactoside* in 1 studies

Other Studies

1 other study(ies) available for bromochloroacetic-acid and 5-bromo-4-chloro-3-indolyl-beta-galactoside

Article	Year
Transplantation of a mammary stromal cell line into a mammary fat pad: development of the site-specific in vivo analysis system for mammary stromal cells. Bioscience, biotechnology, and biochemistry, 2011, Volume: 75, Issue:3 The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions. Topics: Adipose Tissue; Animals; Azo Compounds; Carmine; Cell Line; Epithelial Cells; Epithelium; Female; Fibroblasts; Galactosides; Gene Expression; Immunohistochemistry; Indoles; Keratins; Mammary Glands, Animal; Mice; Organoids; Paracrine Communication; Stromal Cells; Transformation, Genetic; Transplantation, Isogeneic	2011

Article

Year

Transplantation of a mammary stromal cell line into a mammary fat pad: development of the site-specific in vivo analysis system for mammary stromal cells.

Bioscience, biotechnology, and biochemistry, 2011, Volume: 75, Issue:3

The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions.

Topics: Adipose Tissue; Animals; Azo Compounds; Carmine; Cell Line; Epithelial Cells; Epithelium; Female; Fibroblasts; Galactosides; Gene Expression; Immunohistochemistry; Indoles; Keratins; Mammary Glands, Animal; Mice; Organoids; Paracrine Communication; Stromal Cells; Transformation, Genetic; Transplantation, Isogeneic

2011