boron and triphosphoric-acid

boron has been researched along with triphosphoric-acid* in 1 studies

Other Studies

1 other study(ies) available for boron and triphosphoric-acid

Article	Year
Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase. Nucleosides, nucleotides & nucleic acids, 2005, Volume: 24, Issue:5-7 In order to introduce pyrimidine C5-propynyl modification into boranophosphate oligodeoxyribonucleotides (BP- ODNs), 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano) triphosphate (d5PUTPalphaB) was synthesized. The two diastereomers were separated by reverse-phase HPLC. Kinetic studies showed that the Rp isomer was a slightly better substrate for MMLV reverse transcriptase than thymidine triphosphate or Rp-thymidine 5'-(alpha-P-borano)triphosphate. Using the Rp isomers of d5PUTPalphaB and the other three 5'-(alpha-P-borano) triphosphates, a DNA primer could be extended to the full length of the template. Topics: Boron; Boron Compounds; DNA; DNA Primers; HIV Reverse Transcriptase; Kinetics; Models, Chemical; Moloney murine leukemia virus; Nucleotides; Oligonucleotides; Polyphosphates; Protein Isoforms; Pyrimidines; RNA-Directed DNA Polymerase; Thymidine; Thymine Nucleotides; Uridine; Uridine Triphosphate	2005

Article

Year

Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase.

Nucleosides, nucleotides & nucleic acids, 2005, Volume: 24, Issue:5-7

In order to introduce pyrimidine C5-propynyl modification into boranophosphate oligodeoxyribonucleotides (BP- ODNs), 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano) triphosphate (d5PUTPalphaB) was synthesized. The two diastereomers were separated by reverse-phase HPLC. Kinetic studies showed that the Rp isomer was a slightly better substrate for MMLV reverse transcriptase than thymidine triphosphate or Rp-thymidine 5'-(alpha-P-borano)triphosphate. Using the Rp isomers of d5PUTPalphaB and the other three 5'-(alpha-P-borano) triphosphates, a DNA primer could be extended to the full length of the template.

Topics: Boron; Boron Compounds; DNA; DNA Primers; HIV Reverse Transcriptase; Kinetics; Models, Chemical; Moloney murine leukemia virus; Nucleotides; Oligonucleotides; Polyphosphates; Protein Isoforms; Pyrimidines; RNA-Directed DNA Polymerase; Thymidine; Thymine Nucleotides; Uridine; Uridine Triphosphate

2005