boron and malic-acid

Aluminum is a major limiting abiotic factor for plant growth and productivity on acidic soils. The primary disorder of aluminum toxicity is the rapid cessation of root elongation. The root apex is the most sensitive part of this organ. Although significant literature evidence and hypothesis exist on aluminum toxicity, the explicit mechanism through which aluminum ceases root growth is still indefinable. The mechanisms of tolerance in plants have been the focus of intense research. Some plant species growing on acidic soils have developed tolerance mechanisms to overcome and mitigate aluminum toxicity, either by avoiding entry of Al

Topics: Acids; Aluminum; Boron; Crops, Agricultural; Genetic Engineering; Malates; Organic Anion Transporters; Plant Roots; Plants; Soil

Boron (B) can alleviate Citrus copper (Cu)-toxicity. However, the underlying mechanism by which B mitigates Cu-toxicity is unclear. 'Xuegan' (Citrus sinensis) seedlings were exposed to 0.5 (control) or 350 (Cu-toxicity) µM Cu and 2.5 or 25 µM B for 24 weeks. Thereafter, we investigated the secretion of low molecular weight compounds [LMWCs; citrate, malate, total soluble sugars (TSS), total phenolics (TP), and total free amino acids (TFAA)] by excised roots and their concentrations in roots and leaves, as well as related enzyme gene expression and activities in roots and leaves. Cu-stress stimulated root release of malate and TFAA, which might contribute to citrus Cu-tolerance. However, B-mediated-mitigation of Cu-stress could not be explained in this way, since B addition failed to further stimulate malate and TFAA secretion. Indeed, B addition decreased Cu-stimulated-secretion of malate. Further analysis suggested that Cu-induced-exudation of malate and TFAA was not regulated by their levels in roots. By contrast, B addition increased malate, citrate, and TFAA concentrations in Cu-toxic roots. Cu-toxicity increased TP concentration in 25 μM B-treated leaves, but not in 2.5 μM B-treated leaves. Our findings suggested that the internal detoxification of Cu by LMWCs played a role in B-mediated-alleviation of Cu-toxicity.

Topics: Amino Acids; Boron; Citrates; Citric Acid; Citrus sinensis; Copper; Exudates and Transudates; Gene Expression; Malates; Phenols; Seedlings

Tolerance to boron (B) is still not completely understood. We tested here the hypothesis that Thellungiella halophila, an Arabidopsis thaliana-related 'extremophile' plant, with abundance of B in its natural environment, is tolerant to B, and examined the potential mechanisms of this tolerance. With 1-10 mm B applied ([B](ext)) to Thellungiella and Arabidopsis grown in hydroponics, the steady-state accumulated B concentration ([B](int)) in the root was below [B](ext), and was similar in both, suggesting both extrude B actively. Whether grown in soil or hydroponically, the shoot [B](int) was higher in Arabidopsis than in Thellungiella, suggesting more effective net B exclusion by Thellungiella root. Arabidopsis exhibited toxicity symptoms including reduced shoot fresh weight (FW), but Thellungiella was not affected, even at similar levels of shoot-accumulated [B](int) (about 10 to 40 mm B in 'shoot water'), suggesting additional B tolerance mechanism in Thellungiella shoot. At [B](ext) = 5 mm, the summed shoot concentration of the potentially B-binding polyhydroxyl metabolites (malic acid, fructose, glucose, sucrose and citric acid) in Arabidopsis was below [B](int) , but in Thellungiella it was over twofold higher than [B](int) , and therefore likely to allow appreciable 1:2 boron-metabolite complexation in the shoot. This, we suggest, is an important component of Thellungiella B tolerance mechanism.

Topics: Arabidopsis; Biomass; Boron; Brassicaceae; Citric Acid; Fructose; Glucose; Hydroponics; Malates; Plant Roots; Plant Shoots; Salt-Tolerant Plants; Soil; Stress, Physiological; Sucrose