bisnoryangonin and hispidin

The phenolic compounds of Inonotus rheades (Pers.) Bondartsev and Singer (Hymenochaetaceae), a typical xylotrophic basidiomycete, and accumulation of styrylpyrones in mycelium under the influence of light of different wavelengths were investigated. Six styrylpyrones (cis- and trans-hispidin, cis- and trans-bisnoryangonin, and phellinins A1 and A2) and 5 bis(styrylpyrones) (3,14'-bishispidinyl, hypholomin B, 3-bisnoryangonyl-14'-hispidin, 1,1-distyrylpyrylethane, and rheadinin) were detected in the extract of I. rheades mycelium using reversed phase ultra-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry (RP-UPLC-DAD-ESI/MS). The results showed that the maximal content of styrylpyrones was observed under the influence of blue light (8.10 mg/g of dry mycelium weight). Moreover, hispidin was the dominant compound in all experimental groups. Pigmentation intensity gradually decreased after shifting the light spectrum into darkness. It can be concluded that cultivation of I. rheades mycelium under the blue part of the light spectrum leads to the accumulation of styrylpyrones that have nutraceutical and medicinal significance.

Topics: Basidiomycota; Chromatography, High Pressure Liquid; Mycelium; Phenols; Plant Extracts; Pyrones; Spectrometry, Mass, Electrospray Ionization; Styrenes

Four metabolites, hispidin, bisnoryangonin, muscimole, and ibotenic acid, from potentially psychoactive mushrooms were analyzed by GLC--mass spectrometry as their trimethylsilyl derivatives. This method was applied to the first two compounds in Gymnopilus punctifolius (Peck) Singer and to the last two compounds in Amania pantherina (Fr.) Secr.

Topics: Agaricales; Amanita; Chromatography, Gas; Ibotenic Acid; Isoxazoles; Mass Spectrometry; Pyrones; Styrenes