bioallethrin and cyfluthrin

The present study evaluated the correlation between the concentrations of polychlorinated biphenyls (PCBs) and pesticides in the follicular fluid (FF) obtained during intracytoplasmic sperm injection (ICSI) with the ovarian response, endometrial thickness, and embryological and clinical outcomes.. Cross-sectional observational study.. Women aged 20 to 38 years (300 infertile couples) presenting to a university-affiliated fertility center were approached to participate in the study. Only 150 couples that underwent ICSI for male factor infertility agreed to participate, and 94 of them had retrieved enough FF samples suitable for laboratory testing. The FF sample was obtained, centrifuged, and stored in liquid nitrogen. Two organochlorine pesticides (OCPs), Lindane and DDT;three organophosphates (OPs);chlorpyrifos;Diazinon; malathion; one Chloroacetanilide (Pretilachlor);two pyrethroids(Bioallethrin and β-cyfluthrin); and the concentrations of four PCBs, 28, 52, 138, 180, were estimated in the obtained FF samples by using gas chromatography/mass spectrometry. SPSS statistical analysis program (version 17) was used for analysis. Multiple regression analysis was used to correlate the PCBs and pesticides with ICSI outcomes.. There were significant negative correlations between FF concentrations of the eight examined pesticides and the four PCBs on the endometrial thickness. However, Pretilachlor, chlorpyrifos, β-cyfluthrin, and Diazinon were the only toxic agents that negatively correlated with the number of the oocytes retrieved. Fertilization and early embryo cleavage rates were negatively correlated with Pretilachlor and β-cyfluthrin. Moreover, high concentrations of Lindane,DDT, Diazinon,and chlorpyrifos were significantly associated lower implantation rate. PCB 28 and 180 concentration in the FF was associated with a lower number of retrieved oocytes and fertilization rate, respectively. The number of implanted embryos was negatively correlated with PCB 52 FF concentration. However, the clinical pregnancy rate did not reach the level of significance.. Higher concentrations of any studied PCBs and pesticides are associated with thinner endometrial thickness. The higher the level of Pretilachlor, β-cyfluthrin, PCB 28 and 180, the lower the retrieval, fertilization, and embryo cleavage rates. High PCB and pesticide concentrations in the FF adversely affected embryological ICSI outcomes. However, more data are needed to evaluate their effect on the clinical outcome.

Topics: Allethrins; Chlorpyrifos; Cross-Sectional Studies; DDT; Diazinon; Environmental Pollutants; Female; Follicular Fluid; Hexachlorocyclohexane; Humans; Malathion; Nitriles; Pesticides; Polychlorinated Biphenyls; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Pyrethrins; Sperm Injections, Intracytoplasmic

Pyrethroids are insecticides with low acute toxicity in mammals but their world-wide use for domestic and occupational purposes has caused concern about the risks of long-term exposure. The mammalian toxicity of pyrethroids is related to disturbances of membrane function in neuronal tissues whereas their influence on nonneuronal tissues is poorly understood. Recently, selected pyrethroids were shown to affect the function of rat synaptosomal and leukocyte membranes similarly. The present investigation explores to what extent their influence on the function of intact cells, i. e. isolated rat mast cells, correlates with these membrane interactions.. Permethrin and the more water soluble esbiol (S-bioallethrin), both type I pyrethroids, and cyfluthrin, type II, used alone and together with the enhancing substance piperonyl butoxide (PBO) at concentration ratios of 1:5 and 1:10, were tested for influence on histamine release induced by compound 48/80 without and with calcium.. Permethrin (5-10 microM) caused a 10-25 % inhibition of the histamine release in the absence of calcium but did not affect the response with calcium present and had no interaction with PBO. Esbiol (10 microM) was an effective inhibitor on its own, with 70 and 45% inhibition in the absence and presence of calcium, respectively, and caused virtually complete inhibition in a synergistic interaction with PBO. The effect of esbiol could partly be ascribed to inhibition of oxidative energy production. Cyfluthrin was inactive at concentrations up to 10 microM. PBO alone (50 microM) caused some inhibition, in particular in the absence of calcium (ca. 25 %).. The results relating to mast cell histamine release reveal both similarities and differences with the influence of the pyrethroids on cell membrane activities. They indicate that not solely membrane interactions but also additional or alternative targets are involved in the effects of pyrethroids on mammalian tissues. Moreover, the pronounced effects of a brief cell exposure suggest that long-term exposure can be hazardous.

Topics: Allethrins; Animals; Energy Metabolism; Histamine Release; In Vitro Techniques; Insecticides; Male; Mast Cells; Nitriles; p-Methoxy-N-methylphenethylamine; Permethrin; Piperonyl Butoxide; Pyrethrins; Rats; Rats, Wistar

Pyrethroids are widely used insecticides of low acute toxicity in mammals but the consequences of long-term exposure are of concern. Their insecticidal action is related to neurotoxicity and, in addition, there are indications of mammalian immunotoxicity. In order to clarify structure-activity relationships of the membrane interactions of pyrethroids, the present study compared the influence of selected pyrethroids, i.e. permethrin and the more water soluble esbiol (S-bioallethrin), both type I, and cyfluthrin, type II, on the Ca(2+)-ATPase activity of rat brain synaptosomes and peritoneal leukocyte membranes. The pyrethroids were tested alone as well as mixed with the enhancing substance piperonyl butoxide (PBO) at concentration ratios of 1:5 and 1:10. At the highest concentration tested, permethrin (10 microM) alone inhibited the ATPase activity of leukocyte membranes by 20%, whereas the synaptosomes were affected less. Esbiol and cyfluthrin alone did not affect either membrane preparation significantly, whereas PBO (50 microM) alone caused 10-15% inhibition. Mixtures of either pyrethroid with PBO inhibited the ATPase activity of both types of membranes (up to 40% inhibition) in a synergistic manner, which always tended to be supra-additive. With esbiol a true potentiation took place. The synergistic interaction between pyrethroid and PBO was most apparent with mixtures of a concentration ratio of 1:5. The ATPase activity of leukocyte membranes tended to be more susceptible to inhibition than that of synaptosomes. The results are in accordance with the assumption that the mammalian toxicity of pyrethroids can be ascribed to a general disturbance of cell membrane function in neuronal tissue. The results indicate that it may also be the case in the immune apparatus.

Topics: Allethrins; Animals; Brain; Calcium-Transporting ATPases; Cell Membrane; Dose-Response Relationship, Drug; Drug Synergism; In Vitro Techniques; Insecticides; Leukocytes; Male; Nitriles; Permethrin; Piperonyl Butoxide; Pyrethrins; Rats; Rats, Wistar; Structure-Activity Relationship; Synaptosomes

Deltacide (S-bioallethrin 0.71% w/v, deltamethrin 0.5% w/v, piperonyl butoxide 8.9% w/v excipients to 100% w/v) and Solfac UL 015 (cyfluthrin 1.5% w/v) were evaluated against the sentinel sugar-fed adults and 4th-instar larvae of Aedes aegypti at 17 storey high-rise apartments in Malaysia using ULV applications. The impact of both insecticides on field populations of Ae. aegypti and Ae. albopictus larvae was monitored weekly using bottle containers. Both Deltacide and Solfac UL 015 showed adulticidal and larvicidal effects. This was the first field trial using Deltacide against dengue vectors in Malaysia and showed its potential for use in dengue vector control programs.

Topics: Aedes; Allethrins; Animals; Dengue; Humans; Insect Vectors; Insecticides; Larva; Malaysia; Nitriles; Pest Control; Piperonyl Butoxide; Pyrethrins