betalains and thiazolyl-blue

betalains has been researched along with thiazolyl-blue* in 1 studies

Other Studies

1 other study(ies) available for betalains and thiazolyl-blue

ArticleYear
Parameters for cellular viability and membrane function in chenopodium cells show a specific response of extracellular pH to heat shock with extreme Q10.
    Plant biology (Stuttgart, Germany), 2006, Volume: 8, Issue:1

    The effect of brief heat shock on Chenopodium cells was investigated by measuring biochemical parameters for cellular vitality, membrane function and integrity: extracellular pH, release of osmotic compounds, phosphatase, protein and betalain, and cellular reduction of DCPIP and MTT. A threshold temperature was found at 45 degrees C, where release of osmotic compounds, protein and betalain, and reduction of DCPIP and MTT indicate loss of vitality. Extracellular pH and an alkaline phosphatase responded 10-20 degrees C below this threshold, suggesting that extracellular alkalinization, and probably the release of a phosphatase, are part of a specific cellular response to abiotic stress induced by heat shock. The extracellular proton concentration did not increase above 45 degrees C: this may indicate equilibration of gradients driving this process or an inactivation of cellular mechanisms responsible for extracellular alkalinization. The response of extracellular pH to heat shock in Chenopodium cell suspensions was fast, i.e., up to +1 pH in 5 min. Addition of the K+/H+ antiporter nigericin to Chenopodium cells caused an extracellular alkalinization similar to heat shock. The heat shock-induced extracellular alkalinization was characterized by Q10 values for distinct ranges of temperature (Q10 of 56 for 24-31 degrees C, 2.3 for 31-42 degrees C, and 1.0 for 42-50 degrees C). To the author's knowledge, the Q10 of 56 is the highest found up to now. These results suggest that extracellular protons are involved in temperature sensing and signalling in plant cells, probably via a channel-mediated pathway.

    Topics: 2,6-Dichloroindophenol; Alkaline Phosphatase; Animals; Betalains; Cell Membrane Permeability; Cell Survival; Cells, Cultured; Chenopodium; Extracellular Fluid; Heat-Shock Response; Humans; Hydrogen-Ion Concentration; Nigericin; Plant Proteins; Temperature; Tetrazolium Salts; Thiazoles

2006