betadex and sodium-sulfide

An analytical method based on CZE to determine lansoprazole enantiomers in pharmaceuticals was developed. The primary factors affecting its separation efficiency, which include chiral selector, pH, buffer concentration, capillary temperature and injection time, were optimised. The best results were obtained by using a background electrolyte consisting of 50 mM phosphate adjusted to pH 2.2, 12 mM beta-CD and 5 mM sodium sulphite, in combination with hydrodynamic injection and a 15 kV separation voltage. Detection limits were calculated from baseline noise and found to be 0.64 mg L(-1) for the R enantiomer and 0.72 mg L(-1) for the S enantiomer. The proposed method was used to analyse three different pharmaceutical preparations with recoveries of 91-102% of the label content.

Topics: 2-Pyridinylmethylsulfinylbenzimidazoles; beta-Cyclodextrins; Drug Stability; Electrophoresis, Capillary; Hydrogen-Ion Concentration; Lansoprazole; Linear Models; Pharmaceutical Preparations; Reproducibility of Results; Sensitivity and Specificity; Stereoisomerism; Sulfides; Temperature

Reaction 6(A),6(B)-di(O-tosyl)-beta-cyclodextrin with Na(2)S in DMF gave the cis-dimer of beta-cyclodextrin in 21% isolated yield while the trans-dimer was not detected.

Topics: beta-Cyclodextrins; Crystallography, X-Ray; Indicators and Reagents; Isomerism; Magnetic Resonance Spectroscopy; Models, Molecular; Spectrometry, Mass, Fast Atom Bombardment; Sulfides