betadex and sodium-carbonate

This chapter outlines protocols to evaluate protein localization, recruitment or phosphorylation levels in cholesterol/sphingolipids-enriched cell membrane domains and recommends experimental designs with pharmacological tolls to evaluate potential cell functions associated with these domains. We emphasize the need for the combination of several approaches towards understanding the protein components and cellular functions attributed to these distinct microdomains.

Topics: Animals; beta-Cyclodextrins; Carbonates; Caveolae; Cell Membrane; Cholesterol; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Membrane Microdomains; Muscle, Smooth, Vascular; Myocytes, Smooth Muscle; Rats; Sphingolipids

Garments with coverage of a large part of the body generate an exceptional opportunity for transferring active ingredient to the skin. A fabric finished with β-cyclodextrin derivatives can play a role as a host for a next active substance as a guest. In this paper, different methods for permanent fixation of Monochlorotriazinyl-β-Cyclodextrin (MCT)-β-CD, on inner side of a denim fabric were investigated as a carrier with the purpose of substance formation inclusion complex with (MCT)-CD hydrophobic cavity. In order to fix MCT-CD on the fabric surface, two methods were investigated: (1) simultaneously or one step- and (2) pre- or two step-carbonating with sodium carbonate. Varying chemical and physical analyses such as gravimetrical and elemental analysis, FTIR spectroscopy, SEM images and fastness properties measurements, were studied to evaluate the quality of the host molecule stabilization on the textiles substrates and distinction of better application method.

Topics: Administration, Cutaneous; beta-Cyclodextrins; Carbonates; Clothing; Cosmetics; Humans; Textiles